Team:Northwestern

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[[Image:LOGOBANNER1.jpg|936px|center|link=http://wikipedia.org|Tech Institute]]
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{|- style="background:#FFFFFF; style="font-size: 87%;" color:#000000" cellpadding="1" cellspacing="1" bordercolor="#000000" width="97%" align="center"
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{|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center"
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!align="center"|[[Team:Northwestern|<span style="color:#000000;">'''Home'''</span>]]
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!align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<span style="color:#000000;">'''Brainstorm'''</span>]]
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!align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]]
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!align="center"|[[Team:Northwestern/Team|<span style="color:#000000;">'''Team'''</span>]]
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!align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<span style="color:#000000;">'''Acknowledgements'''</span>]]
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!align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]]
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!align="center"|[[Team:Northwestern/Project|<span style="color:#000000;">'''Project'''</span>]]
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!align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]]
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!align="center"|[[Team:Northwestern/SideProject|<span style="color:#000000;">'''Side Project'''</span>]]
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!align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]]
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!align="center"|[[Team:Northwestern/Parts|<span style="color:#000000;">'''Parts'''</span>]]
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!align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]]
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!align="center"|[[Team:Northwestern/Notebook|<span style="color:#000000;">'''Notebook'''</span>]]
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!align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]]
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!align="center"|[[Team:Northwestern/Calendar|<span style="color:#000000;">'''Calendar'''</span>]]
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!align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]]
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!align="center"|[[Team:Northwestern/Protocol|<span style="color:#000000;">'''Protocol'''</span>]]
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!align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]]
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!align="center"|[[Team:Northwestern/Safety|<span style="color:#000000;">'''Safety'''</span>]]
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!align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]]
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!align="center"|[[Team:Northwestern/Links|<span style="color:#000000;">'''Links'''</span>]]
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!align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]]
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!align="center"|[[Team:Northwestern/References|<span style="color:#000000;">'''References'''</span>]]
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!align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]]
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!align="center"|[[Team:Northwestern/Media|<span style="color:#000000;">'''Media'''</span>]]
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!align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]]
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!align="center"|[[Team:Northwestern/Contact|<span style="color:#000000;">'''Contact'''</span>]]
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!align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]]
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[[Image:ohgodtimithebacteriaareangry.jpg|936px|center]]
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|'''SCIN - Self-regenerating Chitin INduction'''
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== '''Product Description: Bacterial Skin''' ==
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Chitin, found in the exoskeletons of insects and crustaceans as well as the cell walls of fungi, is one of the most abundant organic polymers in nature. Like keratin in skin, it comprises the protective outer layer of these organisms. Our goal is to generate a layer of chitin from a lawn of bacteria (Escherichia coli) in response to an external molecular cue. This cue induces chitin synthesis (fast) and cell lysis (slow), allowing for a build-up of chitin followed by cell lysis and subsequent release into the top layer of the lawn. Abrasions expose cells to the external cue for self-repair. This would create a regenerative chitin biolayer with potential medical and industrial applications.
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TENSE(?)
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{|
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|align="center"|To find out more, click the links below:
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Our main project is to simulate mammalian skin using chitin in E Coli. The project involves four major components: Lawn Formation, Chitin Synthesis, Bacterial Apoptosis, and lac-Operon Signaling.
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<td><a href="https://2010.igem.org/Team:Northwestern/Project"><img width="220" class="icon" src="https://static.igem.org/mediawiki/2010/e/ee/Projectoverview_copy.jpg"></a></td>
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A thick bacterial lawn was generated by plating ydgG K.O. mutants (acquired from the Keio Collection) in an enriched agar plate. The protein product of ydgG plays an integral role in AI-2 Transport. Also, the ydgG knock-outs exhibit increased motility. Ultimately, these properties allowed for a thick and level lawn.
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<td><a href="https://2010.igem.org/Team:Northwestern/Project/Modeling"><img width="65" class="icon" src="https://static.igem.org/mediawiki/2010/a/a7/NU_modeling.jpg"></a></td>
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<td><a href="https://2010.igem.org/Team:Northwestern/Project/Chassis"><img width="65" class="icon" src="https://static.igem.org/mediawiki/2010/a/a6/Nuchassis.jpg"></a></td>
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<td><a href="https://2010.igem.org/Team:Northwestern/Project/Lac"><img width="65" class="icon" src="https://static.igem.org/mediawiki/2010/1/18/Nulac.jpg"></a></td>
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<td><a href="https://2010.igem.org/Team:Northwestern/Project/Chitin Synthesis"><img width="65" class="icon" src="https://static.igem.org/mediawiki/2010/4/4e/Nuchitin.jpg"></a></td>
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<td><a href="https://2010.igem.org/Team:Northwestern/Project/Apoptosis"><img width="65" class="icon" src="https://static.igem.org/mediawiki/2010/c/cb/Nuapop.jpg"></a></td>
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<td align="center">Modeling</td>
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<td align="center">Chassis</td>
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<td align="center">Induction</td>
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<td align="center">Chitin</td>
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<td align="center">Apoptosis</td>
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|[[Image:PosterChild.jpg|330px|center]]
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Once the lawn was established, a lactose solution was sprayed over the lawn to induce chitin synthesis and apoptosis. Lactose is converted into allolactose which binds to the lacI repressor. Once bound, lacI detaches from DNA to allow RNA Polymerase to begin transcription.
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Chitin Synthesis
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Bacterial Apoptosis
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SIGNIFICANCE:
 
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Chitin itself can be used for:
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wound and burn treatment
 
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hemostatic for orthopedic treatment of broken bones
 
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viscoelastic solutions for ophthamology and orthopedic surgery
 
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abdominal adhesions treatment
 
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as antibacterial and antifungal agents and for treatment of mucous membranes
 
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in tumor therapies
 
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in micro surgery, neurosurgery
 
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for treatment of chronic wounds, ulcers and bleeding (chitin powder)   
 
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(http://www.gmp-chitosan.com/en/products-services/chitin.html)
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|colspan="3"|
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wound healing (CITATION NEEDED)
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== '''Northwestern University''' ==
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Northwestern University in Evanston, IL, combines innovative teaching and pioneering research in a highly collaborative environment that transcends traditional academic boundaries.
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industrial:
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Our lab facilities are located at: Technological Institute 2145 Sheridan Road, Evanston, IL 60208
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food/pharmaceutical thickener, stabilizer, paper-strengthener
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insoluble in water and many organic solvents
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etc
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agricultural, cosmetics, dietary supplement, water treatment (filter), etc
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derivatives such as chitosan have just as many uses
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biodegradable
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structural (hard)
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Contemporary application:
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Internalization and Compartmentalization - and thus sequestering - of particles such as oil
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=='''Side Project: Magnetite Production'''==
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Find out more about [http://www.northwestern.edu Northwestern University]
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Our side project is to synthesize superparamagnetic magnetite nanoparticles in E Coli.
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Find out more about [http://www.mccormick.northwestern.edu/ McCormick School of Engineering]
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Nano-manipulation of magnetite particles, which can lead to a plethora of advances, especially in research technology and biomedical technology.
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Find out more about [http://www.weinberg.northwestern.edu/ Weinberg College of Arts and Sciences]
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Understanding and utilization of magnetotaxis and/or magnetoception in bacteria.
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=='''Sponsors'''==
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Possible production and use of ferrofluid, a paramagnetic or superparamagnetic fluid.
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Ability to create uniform nanoparticles will further nanotechnology. (biotech is nanotech that works)
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[[image:NU.jpg|200px]]        [[image:Weinberg.jpg|200px]]        [[image:Picture1.jpg|200px]]        [[image:NEB.jpg|200px]]      [[image:Promega.jpg|200px]]      [[image:invitrogen.jpg|200px]]        [[image:VWR.jpg|200px]]        [[image:BIF.jpg|200px]]        [[image:EPOCH.jpg|200px]]
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[[image:tit.jpg|400px]]       
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== '''Northwestern University''' ==
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|}
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[http://en.wikipedia.org/wiki/Northwestern_University Northwestern University]
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=='''Sponsors'''==
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Latest revision as of 16:56, 16 February 2011


Tech Institute
Home Brainstorm Team Acknowledgements Project Human Practices Parts Notebook Calendar Protocol Safety Links References Media Contact
Ohgodtimithebacteriaareangry.jpg

SCIN - Self-regenerating Chitin INduction

Chitin, found in the exoskeletons of insects and crustaceans as well as the cell walls of fungi, is one of the most abundant organic polymers in nature. Like keratin in skin, it comprises the protective outer layer of these organisms. Our goal is to generate a layer of chitin from a lawn of bacteria (Escherichia coli) in response to an external molecular cue. This cue induces chitin synthesis (fast) and cell lysis (slow), allowing for a build-up of chitin followed by cell lysis and subsequent release into the top layer of the lawn. Abrasions expose cells to the external cue for self-repair. This would create a regenerative chitin biolayer with potential medical and industrial applications.

To find out more, click the links below:

Modeling Chassis Induction Chitin Apoptosis

PosterChild.jpg


Locations of visitors to this page


Northwestern University

Northwestern University in Evanston, IL, combines innovative teaching and pioneering research in a highly collaborative environment that transcends traditional academic boundaries.

Our lab facilities are located at: Technological Institute 2145 Sheridan Road, Evanston, IL 60208

Find out more about Northwestern University

Find out more about McCormick School of Engineering

Find out more about Weinberg College of Arts and Sciences

Sponsors

NU.jpg Weinberg.jpg Picture1.jpg NEB.jpg Promega.jpg Invitrogen.jpg VWR.jpg BIF.jpg EPOCH.jpg Tit.jpg

Retrieved from "http://2010.igem.org/Team:Northwestern"