"
Team:Newcastle/Growing a liquid culture
From 2010.igem.org
(Difference between revisions)
RachelBoyd (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | + | =Liquid culture= | |
| - | # | + | # Transfer 5 ml of LB broth to a universal tube |
| - | #Grow overnight in 37°C(''E.coli'', ''Bacillus subtilis'' at a lower temperature 20-30°C) | + | # Pick a single colony and innoculate it |
| + | # Grow overnight in 37°C(''E.coli'', ''Bacillus subtilis'' at a lower temperature 20-30°C) with constant shaking | ||
| + | |||
| + | {{Team:Newcastle/footer}} | ||
Revision as of 15:02, 28 July 2010
| |||||||||||||
| |||||||||||||
Liquid culture
- Transfer 5 ml of LB broth to a universal tube
- Pick a single colony and innoculate it
- Grow overnight in 37°C(E.coli, Bacillus subtilis at a lower temperature 20-30°C) with constant shaking
   
|
    
|
