Team:Newcastle/Growing a liquid culture


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Overnight liquid culture

Materials required

  1. Universal tube
  2. Relevant antibiotics
  3. LB broth
  4. Relevant colonies


  1. Transfer 5 ml of LB broth to a universal tube
  2. Pick a single colony and innoculate it
  3. Grow overnight in 37°C(E.coli, Bacillus subtilis at a lower temperature 20-30°C) with constant shaking
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