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Team:Newcastle/Growing a liquid culture
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RachelBoyd (Talk | contribs) (New page: ==Liquid culture== #Add 5ml of LB broth #Grow overnight in 37°C(''E.coli'', ''Bacillus subtilis'' at a lower temperature 20-30°C)) |
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| - | # | + | =Overnight liquid culture= |
| - | #Grow overnight in 37°C(''E.coli'', ''Bacillus subtilis'' at a lower temperature 20-30°C) | + | |
| + | ==Materials required== | ||
| + | # Universal tube | ||
| + | # Relevant antibiotics | ||
| + | # LB broth | ||
| + | # Relevant colonies | ||
| + | |||
| + | ==Procedures== | ||
| + | # Transfer 5 ml of LB broth to a universal tube | ||
| + | # Pick a single colony and innoculate it | ||
| + | # Grow overnight in 37°C(''E.coli'', ''Bacillus subtilis'' at a lower temperature 20-30°C) with constant shaking | ||
| + | |||
| + | {{Team:Newcastle/footer}} | ||
Latest revision as of 15:03, 28 July 2010
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Overnight liquid culture
Materials required
- Universal tube
- Relevant antibiotics
- LB broth
- Relevant colonies
Procedures
- Transfer 5 ml of LB broth to a universal tube
- Pick a single colony and innoculate it
- Grow overnight in 37°C(E.coli, Bacillus subtilis at a lower temperature 20-30°C) with constant shaking
   
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