Team:Newcastle/26 August 2010

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(Difference between revisions)
(Results)
(Conclusion)
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===Conclusion===
===Conclusion===
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We realized that we used the wrong ''rocF'' primer, so we repeat the whole procedure from PCR again.
 
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Revision as of 10:50, 26 August 2010

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Contents

yneA

PCR (Repeat)

Aim

To repeat the PCR that we did yesterday using the correct rocF primers.

Materials and Protocol

Please refer to PCR.


PCR Purification

Aim

To remove unwanted primers, taq polymerase, buffer and salts to obtain pure DNA.

Materials and Protocol

Please refer to PCR purification.


Digestion

Aim

To digest the PCR products of pSB1C3 and yneA from PCR purification.

Materials and Protocol

Please refer to restriction digest.


Gel extraction

Aim

To purify the DNA of yneA and pSB1C3 by extracting the bands from the gel after running gel electrophoresis. Concentration of DNA is then checked with NanoDrop.

Materials and Protocol

Please refer to:

Results

Conclusion

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