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From 2010.igem.org

semana 1 trabajo, prueba

As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.

* Inmunoresponse using Syintetic biology and

* Cryobiology applied to plant tissues.

Contents 1 After selection proces we going to go with a Cryobiology Proyect 2 Week 1 06th September - 10th 2010 2.1 Monday 2.1.1 After discution we conclude the Igem’s vector (vial with green cover) is not enough. 2.1.2 First step transform only the vector to get enough and begin ligations. 2.1.3 We going to transform Psb1C3 from plate. For this: 2.1.4 * Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.

After selection proces we going to go with a Cryobiology Proyect

The final design our expresion’s moduls for the poryect were as follow.

Next Notebook paper is our reference for primers using, ligations and moduls Assambly.

The AFP (Anty freeze protein) was synthesized as below

Week 1 06th September - 10th 2010

Monday

After discution we conclude the Igem’s vector (vial with green cover) is not enough.

First step transform only the vector to get enough and begin ligations.

We going to transform Psb1C3 from plate. For this:

* Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.