Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
As summer project our experimental work begin in August. After intensive brain-storming we finnaly decide between two options.
* Inmunoresponse using Synthetic Biology
* Criobiology applied to plant crops
After a selection process we are going to choose the Criobiology Project
The final design of our expresions moduls for the project were as follow.
Next Notebook paper is our reference for primers using, ligations and moduls Assembly.
The AFP (Antifreeze protein) was synthesized as below
Week #1
06th September - 10th September 2010
Monday
After discution we conclude the Igem’s vector (vial with green cover) is not enough.
First step transform only the vector to get enough and begin ligations.
We are going to transform Psb1C3 from plate. For this:
- Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.
- Make LB agar plates.
- Complete procedure for making quimio and electro-competent cells.
Tuesday
Completed competent cells stored aliquots at -80 degrees each vial with 150μl.
We transformed TOP10 cells with Psb1C3.
- Planned and prepared for tomorrow's transformation