Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
(Difference between revisions)
(→Transformation with PSB1C3) |
(→First step: Transform using only the vector to get enough material.) |
||
Line 39: | Line 39: | ||
- | |||
Line 48: | Line 47: | ||
*''' Some LB agar and 35ug/ml cloramphenicol plates made up . ''' | *''' Some LB agar and 35ug/ml cloramphenicol plates made up . ''' | ||
- | *''' We | + | *''' We al ready made quimio and electro-competent cells for transformation.''' |
*''' We transformed on ''E. coli'' cells strain DH5ALFA with the plasmid.''' | *''' We transformed on ''E. coli'' cells strain DH5ALFA with the plasmid.''' |
Revision as of 19:04, 27 October 2010
As summer project our experimental work began in August, after intensive brainstorming we finnaly decide
between two options.
*Inmunoresponse using Synthetic Biology.
*Cryobiology applied to plant crops.
After a selection process we chose the Cryobiology Project.
The final design of the expressions modules were as follow:
Next Notebook sheet is our reference for primers and ligations and for modules Assembly:
The AFP (Antifreeze Protein) was synthesized as follow:
Week #1
06th September - 10th September 2010
Monday
- We got plasmid PSB1C3 from the Biobrick plate.
- We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.
- Some LB agar and 35ug/ml cloramphenicol plates made up .
- We al ready made quimio and electro-competent cells for transformation.
- We transformed on E. coli cells strain DH5ALFA with the plasmid.