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Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
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==='''First step: Transform using only the vector to get enough material. '''=== | ==='''First step: Transform using only the vector to get enough material. '''=== | ||
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| - | *''' We got plasmid PSB1C3 | + | *''' We got plasmid PSB1C3 from the Biobrick plate. ''' |
*''' We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.''' | *''' We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.''' | ||
Revision as of 19:01, 27 October 2010
As summer project our experimental work began in August, after intensive brainstorming we finnaly decide
between two options.
*Inmunoresponse using Synthetic Biology.
*Cryobiology applied to plant crops.
After a selection process we chose the Cryobiology Project.
The final design of the expressions modules were as follow:
.JPG)
Next Notebook sheet is our reference for primers and ligations and for modules Assembly:
The AFP (Antifreeze Protein) was synthesized as follow:
Week #1
06th September - 10th September 2010
Monday
First step: Transform using only the vector to get enough material.
- We got plasmid PSB1C3 from the Biobrick plate.
- We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.
- Some LB agar and 35ug/ml cloramphenicol plates made up .
- We completed the procedure to make quimio and electro-competent cells.
- We transformed on E. coli cells strain DH5ALFA with the plasmid.
Tuesday
Finished competent cells and stored in aliquots in containers vials at -80 degrees.
We transformed DH5α cells with Psb1C3.
Wednesday
We have not obtained transformats cells.
We checked out the Cloramphenicol
dose and attempt again the transformation using Psb1C3.
Thursday
The primers arrived
and we proceed to amplify the modules by PCR.
