Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
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(→We are going to transform Psb1C3 from plate. To do this:) |
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==='''First step: Transform using only the vector to get enough material. '''=== | ==='''First step: Transform using only the vector to get enough material. '''=== | ||
- | |||
+ | ==='''Transformation with PSB1C3'''=== | ||
- | *''' We | + | *''' We got plasmid PSB1C3 in the Biobrick plate. '' |
- | *''' | + | *''' We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.''' |
+ | |||
+ | *''' Some LB agar and 35ug/ml cloramphenicol plates made up . ''' | ||
*''' We completed the procedure to make quimio and electro-competent cells.''' | *''' We completed the procedure to make quimio and electro-competent cells.''' | ||
+ | *''' We transformed on ''E. coli'' cells strain DH5ALFA with the plasmid.''' | ||
==''Tuesday''== | ==''Tuesday''== |
Revision as of 18:54, 27 October 2010
As summer project our experimental work began in August, after intensive brainstorming we finnaly decide
between two options.
*Inmunoresponse using Synthetic Biology.
*Cryobiology applied to plant crops.
After a selection process we chose the Cryobiology Project.
The final design of the expressions modules were as follow:
Next Notebook sheet is our reference for primers and ligations and for modules Assembly:
The AFP (Antifreeze Protein) was synthesized as follow:
Week #1
06th September - 10th September 2010
Monday
We discussed and concluded that the Igem’s vector quantity is not enough.
First step: Transform using only the vector to get enough material.
Transformation with PSB1C3
- ' We got plasmid PSB1C3 in the Biobrick plate.
- We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.
- Some LB agar and 35ug/ml cloramphenicol plates made up .
- We completed the procedure to make quimio and electro-competent cells.
- We transformed on E. coli cells strain DH5ALFA with the plasmid.