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Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
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__NOTOC__ | __NOTOC__ | ||
| - | '''As summer project our experimental work | + | '''As summer project our experimental work began in August. After intensive brainstorming we finnaly decide''' |
| - | + | '''between two options.''' | |
| - | ''' | + | *''Inmunoresponse using Synthetic Biology'' |
| - | + | *''Criobiology applied to plant crops'' | |
| - | '''The final design of our expresions moduls | + | = '''After a selection process we chose the Criobiology Project''' = |
| + | |||
| + | '''The final design of our expresions moduls is as follow.''' | ||
[[Image:Primers(2).JPG |center|500px||border|caption]] | [[Image:Primers(2).JPG |center|500px||border|caption]] | ||
| - | '''Next Notebook paper is our reference for | + | '''Next Notebook paper is our reference for primer's use, ligations and moduls Assembly.''' |
| Line 33: | Line 35: | ||
==''Monday''== | ==''Monday''== | ||
| - | ===''' | + | ==='''We discussed and concluded the Igem’s vector (vial with green cover) is not enough.'''=== |
| - | ==='''First step transform only | + | ==='''First step transform only vector to get enough and begin ligations.'''=== |
==='''We are going to transform Psb1C3 from plate. For this:'''=== | ==='''We are going to transform Psb1C3 from plate. For this:'''=== | ||
| Line 42: | Line 44: | ||
*'''Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.''' | *'''Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.''' | ||
| - | *''' | + | *''' 10 LB agar and 35ug/ml cloramphenicol plates made up . ''' |
*'''Complete procedure for making quimio and electro-competent cells.''' | *'''Complete procedure for making quimio and electro-competent cells.''' | ||
| Line 49: | Line 51: | ||
==''Tuesday''== | ==''Tuesday''== | ||
| - | ==='''Completed competent cells stored aliquots at -80 degrees each vial with 150μl.'''=== | + | ==='''Completed competent cells and stored aliquots at -80 degrees each vial with 150μl.'''=== |
| - | ==='''We transformed | + | ==='''We transformed DH5α cells with Psb1C3.'''=== |
| - | |||
==''Wednesday''== | ==''Wednesday''== | ||
| - | ==='''For a strange | + | ==='''For a strange reason we have not transformats cells'''=== |
==='''today we are going to check out the Cloramphenicol'''=== | ==='''today we are going to check out the Cloramphenicol'''=== | ||
==='''dose and try again the transformation with Psb1C3.'''=== | ==='''dose and try again the transformation with Psb1C3.'''=== | ||
| Line 68: | Line 69: | ||
[[Image:PCR.gif]] | [[Image:PCR.gif]] | ||
| - | ==='''The amplification is correct and we have to | + | ==='''The amplification is correct and we have to looking for a nanodrop'''=== |
==='''to quantify DNA’s concentrations.'''=== | ==='''to quantify DNA’s concentrations.'''=== | ||
Revision as of 16:37, 26 October 2010
As summer project our experimental work began in August. After intensive brainstorming we finnaly decide
between two options.
*Inmunoresponse using Synthetic Biology
*Criobiology applied to plant crops
After a selection process we chose the Criobiology Project
The final design of our expresions moduls is as follow.
.JPG)
Next Notebook paper is our reference for primer's use, ligations and moduls Assembly.
The AFP (Antifreeze protein) was synthesized as below
Week #1
06th September - 10th September 2010
Monday
We discussed and concluded the Igem’s vector (vial with green cover) is not enough.
First step transform only vector to get enough and begin ligations.
We are going to transform Psb1C3 from plate. For this:
- Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.
- 10 LB agar and 35ug/ml cloramphenicol plates made up .
- Complete procedure for making quimio and electro-competent cells.
Tuesday
Completed competent cells and stored aliquots at -80 degrees each vial with 150μl.
We transformed DH5α cells with Psb1C3.
Wednesday
For a strange reason we have not transformats cells
today we are going to check out the Cloramphenicol
dose and try again the transformation with Psb1C3.
Thursday
In vector’s absence we have recived the primer’s synthesis
and proceed to amplify by PCR.
