From 2010.igem.org

Revision as of 04:22, 26 October 2010

As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.

* Inmunoresponse using Syintetic biology and

* Cryobiology applied to plant tissues.

After selection proces we going to go with a Cryobiology Proyect

The final design our expresion’s moduls for the poryect were as follow.

Next Notebook paper is our reference for primers using, ligations and moduls Assambly.

The AFP (Anty freeze protein) was synthesized as below

Week #1

06th September - 10th September 2010

Monday

After discution we conclude the Igem’s vector (vial with green cover) is not enough.

First step transform only the vector to get enough and begin ligations.

We going to transform Psb1C3 from plate. For this:

• Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.

• Make LB agar plates.

• Complete procedure for making quimio and electro competent cels.

Tuesday

Completed competent cells stored aliquots at -80 degrees each vial with 150μl.

We transformed TOP10 cells with Psb1C3.

• Planned and prepared for tomorrow's transformation

Wednesday

For a strange razon we have not transformats cells

today we going to check out the Cloramphenicol

dose and try again the transformation with Psb1C3.

Thursday

In vector’s absence we have recived the primer’s sintesis

and proceed to amplify by PCR.

The amplification is correct and we have to looking for a nanodrop

to quantify DNA’s concentrations.