"
Team:Mexico-UNAM-CINVESTAV/Notebook/Week One
From 2010.igem.org
(Difference between revisions)
| Line 37: | Line 37: | ||
==='''We going to transform Psb1C3 from plate. For this:'''=== | ==='''We going to transform Psb1C3 from plate. For this:'''=== | ||
| - | + | ||
| + | *'''Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.''' | ||
| + | |||
| + | *''' Make LB agar plates. ''' | ||
| + | |||
| + | *'''Complete procedure for making quimio and electro competent cels.''' | ||
| + | |||
| + | |||
| + | ==''Tuesday''== | ||
| + | |||
| + | ==='''Completed competent cells stored aliquots at -80 degrees each vial with 150μl.'''=== | ||
| + | |||
| + | ==='''We transformed TOP10 cells with Psb1C3.'''=== | ||
| + | |||
| + | |||
| + | |||
| + | ==''Wednesday''== | ||
| + | |||
| + | ==='''For a strange razon we have not transformats cells'''=== | ||
| + | ==='''today we going to check out the Cloramphenicol'''=== | ||
| + | ==='''dose and try again the transformation with Psb1C3.'''=== | ||
Revision as of 03:00, 24 October 2010
As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.
* Inmunoresponse using Syintetic biology and
* Cryobiology applied to plant tissues.
Contents |
After selection proces we going to go with a Cryobiology Proyect
The final design our expresion’s moduls for the poryect were as follow.
.JPG)
Next Notebook paper is our reference for primers using, ligations and moduls Assambly.
The AFP (Anty freeze protein) was synthesized as below
Week 1 06th September - 10th 2010
Monday
After discution we conclude the Igem’s vector (vial with green cover) is not enough.
First step transform only the vector to get enough and begin ligations.
We going to transform Psb1C3 from plate. For this:
- Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.
- Make LB agar plates.
- Complete procedure for making quimio and electro competent cels.
