Team:Mexico-UNAM-CINVESTAV/Notebook/Week One

From 2010.igem.org

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'''As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.'''
'''As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.'''
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  '''* Inmunoresponse using Syintetic biology and
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  '''* Inmunoresponse using Syintetic biology and
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  '''* Cryobiology applied to plant tissues.
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  '''* Cryobiology applied to plant tissues.
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= '''After selection proces we going to go  with a Cryobiology Proyect.''' =
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= '''After selection proces we going to go  with a Cryobiology Proyect''' =
'''The final design our expresion’s moduls for  the poryect were as follow.'''
'''The final design our expresion’s moduls for  the poryect were as follow.'''
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= Week 1  06th  September - 10th  2010 =
= Week 1  06th  September - 10th  2010 =
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= ''Monday'' =
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==''Monday''==
==='''After discution we conclude the Igem’s vector (vial with green cover) is not enough.'''===
==='''After discution we conclude the Igem’s vector (vial with green cover) is not enough.'''===
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==='''We going to  transform Psb1C3  from plate.  For this:'''===
==='''We going to  transform Psb1C3  from plate.  For this:'''===
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===*''''' Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.'''''===

Revision as of 02:32, 24 October 2010

semana 1 trabajo, prueba


As summer proyect our experimental work began at agust after intensive brian storming finaly deccided between two options.

* Inmunoresponse using Syintetic biology and
* Cryobiology applied to plant tissues.

Contents

After selection proces we going to go with a Cryobiology Proyect

The final design our expresion’s moduls for the poryect were as follow.

caption


Next Notebook paper is our reference for primers using, ligations and moduls Assambly.


Primers1.JPG

The AFP (Anty freeze protein) was synthesized as below

Vector001.jpg


Week 1 06th September - 10th 2010

Monday

After discution we conclude the Igem’s vector (vial with green cover) is not enough.

First step transform only the vector to get enough and begin ligations.

We going to transform Psb1C3 from plate. For this:

* Prepare a stock of Cloramphenicol, Kanamicyn, Ampicilin solutions.