Team:MIT mge
From 2010.igem.org
(Difference between revisions)
Line 9: | Line 9: | ||
#topnav li.notebook ul { | #topnav li.notebook ul { | ||
display: block; | display: block; | ||
- | } | + | }#content { |
+ | background-image: url('http://www.allbestwallpapers.com/wallpaper/flower/image/blue_hydrangea_flower,_close-up_photography.jpg'); | ||
+ | } | ||
</style> | </style> | ||
</head> | </head> | ||
Line 15: | Line 17: | ||
<div class="header"> | <div class="header"> | ||
- | <div style="width: | + | |
- | < | + | <div style="width:250px; margin: 10px; position: relative; top: -4px; left:-11px; display: block; float:right; padding: 7px; background-color: white;"> |
- | + | <dl id="nav"> | |
- | + | <dt><b>Bacterial Protocol</b></dt> | |
- | + | <dd> | |
- | + | <ul> | |
- | + | <li><a href="https://2010.igem.org/Team:MIT_bconst">Biobrick Construction</a></li> | |
- | + | <li><a href="https://2010.igem.org/Team:MIT_bexp">Bacterial Experiments</a></li> | |
- | + | </ul> | |
- | + | </dd> | |
- | </ | + | <dt><b>Mammalian Protocol</b></dt> |
- | < | + | |
+ | <dd> | ||
+ | <ul> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_mmethods">Microfluidics</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_mge">Genetic Engineering</a></li> | ||
+ | <li><a href="https://2010.igem.org/Team:MIT_gateway">Gateway Cloning</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </dd> | ||
+ | |||
+ | </dl> | ||
</div> | </div> | ||
- | <div id="unique" style="padding: | + | |
- | <table width= | + | <div id="unique" style="padding:0px; font-size: 14px; border: 1px solid black; margin:0px; background-color:transparent;"> |
+ | <table width=650px style="background-color: white; margin-top:5px; padding: 10px;"> | ||
+ | <tr><td><div class="bodybaby">mammalian genetic engineering protocol</div></td> | ||
<tr><td><br>The Mammalian team created lines of cells using lentiviruses for transfection.<br><br> | <tr><td><br>The Mammalian team created lines of cells using lentiviruses for transfection.<br><br> | ||
<div class="outline"> | <div class="outline"> |
Revision as of 16:39, 24 October 2010
mammalian genetic engineering protocol |
The Mammalian team created lines of cells using lentiviruses for transfection. |
Calcium Phosphate Transfection Beforehand:
Protocol
Filter and Concentrate Virus Protocol
Materials HBSS Buffer (500 mL)
Calcium Chloride Solution (50mL)
Freezing medium
Supplemented DMEM
|