Team:MIT mge
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Revision as of 20:03, 16 October 2010
Bacterial Protocol
Mammalian Protocol
Mammalian Protocol
Microfluidics Protocol
Genetic Engineering Protocol
mammalian genetic engineering protocol |
The Mammalian team created lines of cells using lentiviruses for transfection. |
Calcium Phosphate Transfection Beforehand:
Protocol
Filter and Concentrate Virus Protocol
Materials HBSS Buffer (500 mL)
Calcium Chloride Solution (50mL)
Freezing medium
Supplemented DMEM
|