Team:MIT k415032

From 2010.igem.org

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<center><a href="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png"></a></center><br><br>
<center><a href="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png"></a></center><br><br>
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<a href="http://2010.igem.org/Team:MIT_k415031"><b>&larr; K415031</b></a><a style="float:right;" href="http://2010.igem.org/Team:MIT_original"><b>All Original Parts &rarr;</b></a>
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<a href="http://2010.igem.org/Team:MIT_k415031"><b>&larr; K415031</b></a><a style="float:right;" href="http://2010.igem.org/Team:MIT_k415108"><b>K415108 &rarr;</b></a>
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Latest revision as of 02:44, 27 October 2010

K415031

From the 2010 MIT iGEM Team.See the Parts Registry Page!! →

Improved PLux/cI-OR Promoter
This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.

Slightly weaker binding than K415031.



← K415031K415108 →