Team:Lethbridge

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<p>University of Lethbridge IGEM team</p>
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<p>&nbsp;</p>
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    <li class="TabbedPanelsTab" tabindex="0">Main</li>
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    <li class="TabbedPanelsTab" tabindex="0">Team</li>
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    <li class="TabbedPanelsTab" tabindex="0">Projects</li>
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    <li class="TabbedPanelsTab" tabindex="0">Notebook</li>
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    <li class="TabbedPanelsTab" tabindex="0">Meetings</li>
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    <li class="TabbedPanelsTab" tabindex="0">Parts</li>
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    <div class="TabbedPanelsContent">blah balh balh</div>
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      <p>Our team is based yada yada</p>
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          <li class="TabbedPanelsTab" tabindex="0">Team Pics</li>
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          <li class="TabbedPanelsTab" tabindex="0">Tab 3</li>
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          <li class="TabbedPanelsTab" tabindex="0">Team bios</li>
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          <div class="TabbedPanelsContent">Team pictures</div>
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          <div class="TabbedPanelsContent">Content 3</div>
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          <div class="TabbedPanelsContent">Pics of us</div>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>&nbsp;</p>
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      <p>Overview</p>
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          <li class="TabbedPanelsTab" tabindex="0">Project 1</li>
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        <div class="TabbedPanelsContentGroup">
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          <div class="TabbedPanelsContent">Content 1</div>
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          <div class="TabbedPanelsContent">Content 2</div>
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      <p>&nbsp;</p>
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<b><font size=+1>Jump to Month:<br>
 
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<a href=#april>April</a><br>
 
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<a href=#may>May</a><br></b><br>
 
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<a name="april"></a></font>
 
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<b><font size=+2>April 13/2010</font> (In the Lab: JV, AS)</b><br>
 
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<b>Objective:</b> Test Restriction Endonucleases for Activity<br>
 
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<b>Relevant Information:</b><br>
 
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Endonucleases available
 
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<table><table border="3">
 
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<tr><td>Endonuclease</td><td>Optimal Buffer**</td><td>Other Buffers</td></tr>
 
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<tr><td>EcoRV</td><td>None</td><td>2xT(100%); O,G(50-100%)</td></tr>
 
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<tr><td>EcoRI</td><td>Red</td><td>O(100%);R(100%)*;2xT(100%)</td></tr>
 
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<tr><td>BcuI/SpeI</td><td>Tango</td><td>B(50-100%);G(50-100%)</td></tr>
 
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<tr><td>XbaI</td><td>Tango</td><td>B,G,2xT(50-100%)</td></tr>
 
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<tr><td>PstI</td><td>Orange</td><td>R(100%); B,G,T,2xT(50-100%)</td></tr>
 
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<tr><td>DpnI</td><td>Tango</td><td>B,G(100%): O,R,2xT(50-100%)</td></tr>
 
</table>
</table>
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*Star Activity<br>
 
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**Optimal Buffer from Fermentas<br><br>
 
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Use pUC19 plasmid as test, it has cut sites for EcoRI, PstI, XbaI (unsure about BcuI/SpeI, DpnI but will try anyways), and none for EcoRV <br>
 
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<u>Red Buffer:</u> EcoRI, PstI, Control (No Enzyme)<br>
 
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<u>Tango Buffer:</u> BcuI/SpeI, XbaI, DpnI, Control (No Enzyme><br><br>
 
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<u>Methods:</u>
 
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Set up Master Mixes:
 
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<table><table border="3">
 
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<tr><td><b>Red MM</b></td><td>per tube (&micro;L)</td><td>Total (&micro;L)</td></tr>
 
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<tr><td>MilliQ H<sub>2</sub>0</td><td>13.75</td><td>55</td></tr>
 
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<tr><td>Red Buffer (10x)</td><td>2</td><td>7</td></tr>
 
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<tr><td>pUC19 (10pg/&micro;L)</td><td>2</td><td>7</td></tr>
 
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<tr><td><b>Total</b></td><td>19.75</td><td>69</td></tr>
 
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</table><br>
 
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<table><table border="3">
 
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<tr><td><b>Tango MM</b></td><td>per tube (&micro;L)</td><td>Total (&micro;L)</td></tr>
 
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<tr><td>MilliQ H<sub>2</sub>0</td><td>13.75</td><td>55</td></tr>
 
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<tr><td>Tango Buffer (10x)</td><td>2</td><td>7</td></tr>
 
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<tr><td>pUC19 (10pg/&micro;L)</td><td>2</td><td>7</td></tr>
 
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<tr><td><b>Total</b></td><td>19.75</td><td>69</td></tr>
 
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</table><br>
 
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To each tube, add <b>19.75&micro;L</b> of master mix and <b>0.25&micro;L</b> of enzyme<br>
 
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Incubated reaction mixes at 37<sup>o</sup>C (Start:7:00pm; End:7:45pm)<br>
 
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Add 3.3&micro;L of 6x loading dye to each reaction mixture and load 10&micro;L final volume onto a 1% agarose (in TAE) gel.<br>
 
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Add 1&micro;L of 6x loading dye to 1&micro;L of GeneRuler 1kb ladder (at 0.5&micro;g/&micro;L)<br>
 
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Gel loading order as follows:<br>
 
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<table><table border="3">
 
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<tr><td><b>Lane</b></td><td><b>Sample</b></td></tr>
 
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<tr><td>1</td><td>1kb Ladder</td></tr>
 
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<tr><td>2</td><td>Tango Control</td></tr>
 
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<tr><td>3</td><td>DpnI (Tango)</td></tr>
 
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<tr><td>4</td><td>BcuI/SpeI (Tango)</td></tr>
 
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<tr><td>5</td><td>XbaI (Tango)</td></tr>
 
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<tr><td>6</td><td>EcoRI (Red)</td></tr>
 
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<tr><td>7</td><td>PstI (Red)</td></tr>
 
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<tr><td>8</td><td>Red Control</td></tr>
 
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<tr><td>9</td><td>Empty</td></tr>
 
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<tr><td>10</td><td>Empty</td></tr>
 
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</table><br>
 
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Ran gel at 100V for 1 hour<br>
 
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<b>Results:</b> pUC19 plasmid DNA not present at a high enough concentration to visualize by ethidium bromide staining (1kb ladder did stain). <br>
 
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<b>Conclusion:</b> Will have to re-run experiment with DNA that is present at high enough concentrations to visualize by ethidium bromide staining<br><br><br>
 
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<a name="may"></a>
 
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<b><font size=+2>May 5/2010</font>(in the lab: JV)</b><br>
 
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<b>Objective:</b> Test Restriction Endonucleases for activity (take 2)<br>
 
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<b>Relevant Information:</b><br>
 
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Plasmid DNA used here will be "ES-pSB-CEYFP" from last year's plasmid stocks<br>
 
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Prefix Enzymes are: EcoRI and XbaI<br>
 
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Suffix Enyzmes are: SpeI and PstI<br>
 
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(JV worked out in lab notebook which buffers would be best for each prefix/suffix enzyme combination)<br>
 
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Reactions will be assembled as follows:<br>
 
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<table><table border="3">
 
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<tr><td><b>Enzyme</td><td>Buffer</td><td>Volume MM(&micro;L)</td><td>Volume Enzyme(&micro;L)</td></tr></b>
 
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<tr><td>PstI</td><td>Red</td><td>19.75</td><td>.25</td></tr>
 
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<tr><td>XbaI</td><td>Tango</td><td>19.75</td><td>.25</td></tr>
 
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<tr><td>SpeI</td><td>Tango</td><td>19.75</td><td>.25</td></tr>
 
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<tr><td>EcoRI</td><td>Red</td><td>19.75</td><td>.25</td></tr>
 
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<tr><td>EcoRI/SpeI</td><td>Red</td><td>19.5</td><td>.25+.25</td></tr>
 
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<tr><td>XbaI/SpeI</td><td>Tango</td><td>19.5</td><td>.25+.25</td></tr>
 
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<tr><td>EcoRI/PstI</td><td>Red</td><td>19.5</td><td>.25+.25</td></tr>
 
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<tr><td>XbaI/PstI</td><td>Tango</td><td>19.5</td><td>.25+.25</td></tr>
 
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</table><br>
 
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Make up Master Mixes as follows:<br>
 
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<table><table border="3">
 
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<tr><td><b>Red MM</td><td>per tube(&micro;L)</td><td>Total*(&micro;L)</td></tr></b>
 
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<tr><td>MilliQ H<sub>2</sub>0</td><td>15.75</td><td>86.675</td></tr>
 
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<tr><td>Red Buffer (10x)</td><td>2</td><td>11</td></tr>
 
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<tr><td>pDNA**</td><td>2</td><td>11</td></tr>
 
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</table><br>
 
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<table><table border="3">
 
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<tr><td><b>Tango MM</td><td>per tube(&micro;L)</td><td>Total*(&micro;L)</td></tr></b>
 
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<tr><td>MilliQ H<sub>2</sub>0</td><td>15.75</td><td>86.675</td></tr>
 
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<tr><td>Tango Buffer (10x)</td><td>2</td><td>11</td></tr>
 
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<tr><td>pDNA**</td><td>2</td><td>11</td></tr>
 
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</table><br>
 
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*Volume per reaction time 5.5
 
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**Unknown concentration of pDNA<br><br>
 
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Incubated for 70min at 37<sup>o</sup>C (Start-1:05pm; End-2:15pm)<br>
 
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Added 3.3&micro;L of 6x loading dye to each reaction mixture and loaded 10&micro;L onto a 1% agarose gel (in TAE)<br>
 
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Added 1&micro;L of 6x loading dye to 2&micro;L of gene ruler 1kb ladder<br>
 
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Load order as follows:<br>
 
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<table><table border="3">
 
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<tr><td><b>Lane</td><td>Sample</td><td>Volume Loaded (&micro;L)</td></tr></b>
 
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<tr><td>1</td><td>pSB-CEYFP/PstI</td><td>10</td></tr>
 
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<tr><td>2</td><td>pSB-CEYFP/EcoRI</td><td>10</td></tr>
 
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<tr><td>3</td><td>pSB-CEYFP/EcoRI/PstI</td><td>10</td></tr>
 
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<tr><td>4</td><td>pSB-CEYFP/EcoRI/SpeI</td><td>10</td></tr>
 
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<tr><td>5</td><td>pSB-CEYFP/XbaI/PstI</td><td>10</td></tr>
 
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<tr><td>6</td><td>pSB-CEYFP/XbaI</td><td>10</td></tr>
 
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<tr><td>7</td><td>pSB-CEYFP/SpeI</td><td>10</td></tr>
 
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<tr><td>8</td><td>pSB-CEYFP/XbaI/SpeI</td><td>10</td></tr>
 
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<tr><td>9</td><td>pSB-CEYFP/Red Master Mix Control</td><td>10</td></tr>
 
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<tr><td>10</td><td>pSB-CEYFP/Tango Master Mix Control</td><td>10</td></tr>
 
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<tr><td>11</td><td>pSB-CEYFP/MilliQ H<sub>2</sub>0 Control</td><td>10</td></tr>
 
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<tr><td>12</td><td>Ladder</td><td>4</td></tr>
 
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</table><br>
 
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Ran gel at 100V for 1 hour<br><br>
 
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<b>Results:</b><br>
 
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<a href="https://static.igem.org/mediawiki/2010/thumb/d/dc/100505JV-EnzymeTest1.JPG/800px-100505JV-EnzymeTest1.JPG"><img src="https://static.igem.org/mediawiki/2010/thumb/d/dc/100505JV-EnzymeTest1.JPG/800px-100505JV-EnzymeTest1.JPG"  width="225" height="151"/></a><br>
 
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This gel shows that SpeI does not cut on its own, and does not cut when combined with other enzymes<br>
 
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<b>Conclusion:</b> Test other source of SpeI to see if it has any activity.<br><br>
 
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<b><font size=+2>May 6/2010</font>(in the lab:KG, AS)</b><br>
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<br>
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<b>Objective:</b> To check if the old SpeI enzyme (exp date: March 2011) will cleave plasmid DNA, since we believe the newer SpeI enzyme (exp date: 2012) does not.
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<a href="https://2010.igem.org/Team:Lethbridge">
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<img src="https://static.igem.org/mediawiki/2010/2/22/UofLHome.jpg" width="80"/>
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<br>May 13<br>
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<th><a href="https://2010.igem.org/Team:Lethbridge/Team">
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<img src="https://static.igem.org/mediawiki/2010/0/0d/UofLTeam.jpg" width="80"/>
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<th><a href="https://2010.igem.org/Team:Lethbridge/Project">
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<th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work">
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<th><a href="https://2010.igem.org/Team:Lethbridge/Parts">
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<th><a href="https://2010.igem.org/Team:Lethbridge/Modeling">
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<p>&nbsp;</p>
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<img src="https://static.igem.org/mediawiki/2010/e/e1/UofLModelingbutton.jpg" width="80"/>
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<p>&nbsp; </p>
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Check out these important project links!
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<BLOCKQUOTE>
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=<font color="white">Project Description=
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The tailings ponds that result from the extraction of heavy crude oil and bitumen (used to make synthetic crude oil) from the oil sands have used up vast amounts of fresh water and contain substantial quantities of useable organic matter. While most of the bitumen is extracted from the oil sands, some is left behind and added to the tailings ponds. The residual hydrocarbon compounds can be potentially extracted from the ponds and utilized as another source of fuel. Consequently, cleaning the tailings ponds and increasing efficiency of extraction from the oil sands.
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<br>
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We wish to develop and characterize a BioBrick, that can breakdown some of the more prominent toxic organic compounds found in the tailing ponds to a more useable form.  We are currently targeting catechol, a aromatic compound shown to be degraded by bacteria living in the tailings ponds (Kato <i>et al.</i>, 2001).  Catechol is being converted into 2-hydroxymuconic semialdehyde, which we later hope to further convert into a useful hydrocarbon compound.
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<br>
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Additionally, we plan to target our <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Catechol_Degradation"><font color="#00DC00">catechol degrading enzyme</font></a></html> into a <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Compartamentalization"><font color="#00DC00"> microcompartment</font></a></html> which the <html><a href="https://2009.igem.org/Team:Lethbridge" target="new"><font color="#00DC00"> Lethbridge 2009</font></a></html> team began the work on.  By compartmentalizing the converted catechol, were trying to develop a way of easily removing the useful hydrocarbon product from the tailings ponds.  As a proof of principle, we will target the catechol degradation enzyme into the negatively charged microcompartment by the use of a poly-arginine tag.  Furthermore, to avoid adding a new species into the oil sands environment we plan on using the <html><a href="https://2010.igem.org/Team:Lethbridge/Project/DNA_Degradation"><font color="#00DC00"> DNA digestion part</font></a></html> created by <html><a href="https://2007.igem.org/Berkeley_UC" target="new"><font color="#00DC00"> Berkley in 2007</font></a></html> to render our <i>Escherichia coli </i>cells unable to reproduce or able to horizontally transfer its genes.
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<br>
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Finally, we will be continuing to explore the novel method of the mass production of <html><a href="https://2010.igem.org/Team:Lethbridge/Project/Magnetic_Nanoparticles"><font color="#00DC00"> uniform iron nanoparticles</font></a></html>, which is more efficient and cost effective than current methods (Prozorov <i>et al.</i>, 2007).  To optimize the production of nanoparticles we are attaching signal peptide sequences to export the protein to different areas of the cell.  By attaching these signal peptides and having the protein directed to certain areas of the cell we hope to find which area is most productive to produce nanoparticles.
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<br>
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Reference:<br>
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Kato, T., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001). Isolation and characterization of psychrotrophic bacteria from oil-reservoir and oil sands. <i>Applied Microbial Biotechnology.</i> 55, 794-800.
 +
<br>
 +
Prozorov, T., Mallapragada, S. K., Narasimhan, B., Wang, L., Palo, P., Nilsen-Hamilton, M., Williams, T. J., Bazylinski, D. A., Prozorov, R., and Canfield, P. C. (2007). Protein-mediated synthesis of uniform superparamagnetic magnetite nanocrystals. Adv. Funct. Mater. Advanced Functional Materials. 17, 951-957
 +
 +
=<font color="white">Sponsors=
 +
 +
==<font color="white">Platinum==
 +
 +
===<font color="white">Alberta Innovates Technology Futures===
 +
<html>
 +
<center>
 +
<table>
 +
<tr>
 +
<th>
 +
<a href="http://www.albertainnovates.ca/technology/introduction" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/d/db/UofLAITFlogo.jpg" width="300"/>
 +
</a>
 +
</th>
 +
</tr>
 +
</table>
 +
</center>
 +
</html>
 +
 +
===<font color="white">Oil Sands Initiative===
 +
<b>https://2010.igem.org/Oil_Sands</b>
 +
 +
<html>
 +
<center>
 +
<table border="0" cellpadding="5" cellspacing="5" width="40%">
 +
<tr>
 +
<th>
 +
<a href="http://www.see.ualberta.ca/OSRIN.cfm" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/a/a1/UofLOSRINlogo.jpg" width="250"/>
 +
</a>
 +
</th>
 +
<th><a href="http://www.iseee.ca" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/6/6c/UofLISEEElogo.jpg" width="550"/>
 +
</a></th>
 +
</table>
 +
</center>
 +
</html>
 +
 +
<html>
 +
<center>
 +
<table border="0" cellpadding="5" cellspacing="5" width="95%">
 +
<tr>
 +
<th>
 +
<a href="http://www.conocophillips.ca" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/4/42/UofLConocoPhillipslogo.jpg" width="160"/>
 +
</a>
 +
</th>
 +
<th><a href="http://www.nexeninc.com" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/2/2e/UofLNexenlogo.jpg" width="120"/>
 +
</a>
 +
</th>
 +
<th><a href="http://www.statoil.com" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/4/45/UofLStatoillogo.jpg" width="90"/>
 +
</a>
 +
</th>
 +
<th><a href="http://www.suncor.com" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/c/c3/UofLSuncorlogo.jpg" width="130"/>
 +
</a>
 +
</th>
 +
<th><a href="http://www.total-ep-canada.com" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/e/ed/UofLTotallogo.jpg" width="75"/>
 +
</a>
 +
</th>
 +
</table>
 +
</center>
 +
</html>
 +
 +
==<font color="white">Gold==
 +
 +
 +
===<font color="white">Autodesk===
 +
<html>
 +
<center>
 +
<table>
 +
<tr>
 +
<th>
 +
<a href="http://www.autodeskresearch.com/" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/6/67/UofLAutodesklogo.jpg"/>
 +
</a>
 +
</th>
 +
</tr>
 +
</table>
 +
</center>
 +
</html>
 +
 +
 +
==<font color="white">Silver==
 +
 +
 +
===<font color="white">MathWorks===
 +
<html>
 +
<center>
 +
<table>
 +
<tr>
 +
<th>
 +
<a href="http://www.mathworks.com/" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/c/c3/UofLMathWorkslogo.jpg" width="300" border="0"/>
 +
</a>
 +
</th>
 +
</tr>
 +
</table>
 +
</center>
 +
</html>
 +
 +
 +
===<font color="white">Geneious===
 +
<html>
 +
<center>
 +
<table>
 +
<tr>
 +
<th>
 +
<a href="http://www.geneious.com/" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/3/30/UofLGeneiouslogo.jpg" width="300"/>
 +
</a>
 +
</th>
 +
</tr>
 +
</table>
 +
</center>
 +
</html>
 +
 +
 +
==<font color="white">Bronze==
 +
 +
===<font color="white">Integrated DNA Technologies===
 +
<html>
 +
<center>
 +
<table>
 +
<tr>
 +
<th>
 +
<a href="http://www.idtdna.com/" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/4/43/UofLIDTlogo.jpg"/>
 +
</a>
 +
</th>
 +
</tr>
 +
</table>
 +
</center>
 +
</html>
 +
 +
 +
===<font color="white">GeneArt & Mr. Gene===
 +
<html>
 +
<center>
 +
<align="left">
 +
<table border="0" cellpadding="5" cellspacing="5" width="40%">
 +
<tr>
 +
<th>
 +
<a href="http://www.geneart.com/" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/e/ed/UofLGENEARTlogo.jpg"/>
 +
</a>
 +
</th>
 +
<th><a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/2/26/UofLMRGENElogo.jpg"/>
 +
</a></th>
 +
</table>
 +
</center>
 +
</html>
 +
 +
 +
===<font color="white">Macrogen===
 +
<html>
 +
<center>
 +
<table>
 +
<tr>
 +
<th>
 +
<a href="http://www.macrogen.com/eng/macrogen/macrogen_main.jsp" target="_blank">
 +
<img src="https://static.igem.org/mediawiki/2010/a/a7/UofLMacrogenlogo.jpg"/>
 +
</a>
 +
</th>
 +
</tr>
 +
</table>
 +
</center>
 +
</html>
 +
 +
==<font color="white">Sponsorship Breakdown==
 +
===<font color="white">The University of Lethbridge iGEM Team requests your support in the following ways:===
 +
 +
a) $2000 scholarship of one team member – It is our hope that every team member will be able to travel to the iGEM competition taking place in November 2010.  However, without enough funding, this will not be the situation.  By sponsoring one student at a time, we can work towards reaching our goal of the entire U of L team traveling to MIT and provide every student with the possibility to meet and talk to some of the brightest and inspiring minds of synthetic biology.
 +
 +
b) Monetary donation towards lab supplies, travel arrangements and iGEM competition registration fees.
 +
 +
c) Tangible donations of lab supplies or other necessary materials.
 +
 +
===<font color="white">Donations can be made in two ways:===
 +
 +
1. Donations can be directed towards the U of L iGEM Scholarship account.
 +
 +
2. Donations can be directed towards the U of L iGEM General account and in recognition of your generous support, the U of L iGEM team would like to recognize you and/or your business as outlined in the sponsorship levels.
 +
 +
===<font color="white">Sponsorship Levels===
 +
 +
Platinum - $5000+ or gift in kind <br>
 +
Logo on team shirts, large logo on scientific poster, large logo on team wiki and verbal recognition during team project presentations/media interviews.
 +
 +
Gold - $2000-$4999 or gift in kind<br>
 +
Logo on team shirts, medium logo on scientific poster, medium logo on team wiki and written recognition at end of team project presentations.
 +
 +
Silver - $1000-$1999 or gift in kind<br>
 +
Medium logo on team shirts, scientific poster, medium logo on team wiki and written recognition at end of team project presentations.
 +
 +
Bronze - <$999 or gift in kind<br>
 +
Small logo on team shirts, scientific poster, small logo on team wiki and written recognition at end of team project presentations.
 +
<br>
 +
<br>
 +
<br>

Latest revision as of 15:20, 27 October 2010




Check out these important project links!


Contents

Project Description

The tailings ponds that result from the extraction of heavy crude oil and bitumen (used to make synthetic crude oil) from the oil sands have used up vast amounts of fresh water and contain substantial quantities of useable organic matter. While most of the bitumen is extracted from the oil sands, some is left behind and added to the tailings ponds. The residual hydrocarbon compounds can be potentially extracted from the ponds and utilized as another source of fuel. Consequently, cleaning the tailings ponds and increasing efficiency of extraction from the oil sands.

We wish to develop and characterize a BioBrick, that can breakdown some of the more prominent toxic organic compounds found in the tailing ponds to a more useable form. We are currently targeting catechol, a aromatic compound shown to be degraded by bacteria living in the tailings ponds (Kato et al., 2001). Catechol is being converted into 2-hydroxymuconic semialdehyde, which we later hope to further convert into a useful hydrocarbon compound.

Additionally, we plan to target our catechol degrading enzyme into a microcompartment which the Lethbridge 2009 team began the work on. By compartmentalizing the converted catechol, were trying to develop a way of easily removing the useful hydrocarbon product from the tailings ponds. As a proof of principle, we will target the catechol degradation enzyme into the negatively charged microcompartment by the use of a poly-arginine tag. Furthermore, to avoid adding a new species into the oil sands environment we plan on using the DNA digestion part created by Berkley in 2007 to render our Escherichia coli cells unable to reproduce or able to horizontally transfer its genes.

Finally, we will be continuing to explore the novel method of the mass production of uniform iron nanoparticles, which is more efficient and cost effective than current methods (Prozorov et al., 2007). To optimize the production of nanoparticles we are attaching signal peptide sequences to export the protein to different areas of the cell. By attaching these signal peptides and having the protein directed to certain areas of the cell we hope to find which area is most productive to produce nanoparticles.


Reference:
Kato, T., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001). Isolation and characterization of psychrotrophic bacteria from oil-reservoir and oil sands. Applied Microbial Biotechnology. 55, 794-800.
Prozorov, T., Mallapragada, S. K., Narasimhan, B., Wang, L., Palo, P., Nilsen-Hamilton, M., Williams, T. J., Bazylinski, D. A., Prozorov, R., and Canfield, P. C. (2007). Protein-mediated synthesis of uniform superparamagnetic magnetite nanocrystals. Adv. Funct. Mater. Advanced Functional Materials. 17, 951-957

Sponsors

Platinum

Alberta Innovates Technology Futures

Oil Sands Initiative

https://2010.igem.org/Oil_Sands

Gold

Autodesk


Silver

MathWorks


Geneious


Bronze

Integrated DNA Technologies


GeneArt & Mr. Gene


Macrogen

Sponsorship Breakdown

The University of Lethbridge iGEM Team requests your support in the following ways:

a) $2000 scholarship of one team member – It is our hope that every team member will be able to travel to the iGEM competition taking place in November 2010. However, without enough funding, this will not be the situation. By sponsoring one student at a time, we can work towards reaching our goal of the entire U of L team traveling to MIT and provide every student with the possibility to meet and talk to some of the brightest and inspiring minds of synthetic biology.

b) Monetary donation towards lab supplies, travel arrangements and iGEM competition registration fees.

c) Tangible donations of lab supplies or other necessary materials.

Donations can be made in two ways:

1. Donations can be directed towards the U of L iGEM Scholarship account.

2. Donations can be directed towards the U of L iGEM General account and in recognition of your generous support, the U of L iGEM team would like to recognize you and/or your business as outlined in the sponsorship levels.

Sponsorship Levels

Platinum - $5000+ or gift in kind
Logo on team shirts, large logo on scientific poster, large logo on team wiki and verbal recognition during team project presentations/media interviews.

Gold - $2000-$4999 or gift in kind
Logo on team shirts, medium logo on scientific poster, medium logo on team wiki and written recognition at end of team project presentations.

Silver - $1000-$1999 or gift in kind
Medium logo on team shirts, scientific poster, medium logo on team wiki and written recognition at end of team project presentations.

Bronze - <$999 or gift in kind
Small logo on team shirts, scientific poster, small logo on team wiki and written recognition at end of team project presentations.