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Team:Kyoto/Project/Goal C
From 2010.igem.org
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We used three types of E. coli, E. coli KRX transformed with <partinfo>BBa_K358021</partinfo>, KRX transformed with <partinfo>BBa_K358024</partinfo>, KRX transformed with <partinfo>BBa_K358022</partinfo>. | We used three types of E. coli, E. coli KRX transformed with <partinfo>BBa_K358021</partinfo>, KRX transformed with <partinfo>BBa_K358024</partinfo>, KRX transformed with <partinfo>BBa_K358022</partinfo>. | ||
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'''Measurement''' | '''Measurement''' | ||
Revision as of 16:13, 25 October 2010
Contents |
Goal C: Characterization of the anti-killer gene
Introduction
We checked the function of the anti-killer gene. E.coli transformed with the constructs below was grown in medium without IPTG and IPTG was added to the culture at proper time. The A550 of the culture was measured and the result was compared with that of the experiment of the killer gene in order to find whether the anti-killer gene works correctly.
Method
Bacterial strains
We used three types of E. coli, E. coli KRX transformed with <partinfo>BBa_K358021</partinfo>, KRX transformed with <partinfo>BBa_K358024</partinfo>, KRX transformed with <partinfo>BBa_K358022</partinfo>.
Measurement
We picked up three colonies from each plate, and cultivated them in M9 medium overnight. The overnight cultures were diluted to 0.1-0.12 (OD600) by M9 medium, and we measure OD600 of the culture at several points.
