Team:Heidelberg/Project/miMeasure
From 2010.igem.org
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[[Image:M12-M22_HeLa_daten.jpg|thumb|500px|center|'''GFP/BFP ratio normalized by the negative control''' The data are generated by confocal microscopy of Hela cells, which were transfected with different miMeasure constructs M12-M22 including the negative control (miMeasure construct without binding site). The negative control equals to 1.]] | [[Image:M12-M22_HeLa_daten.jpg|thumb|500px|center|'''GFP/BFP ratio normalized by the negative control''' The data are generated by confocal microscopy of Hela cells, which were transfected with different miMeasure constructs M12-M22 including the negative control (miMeasure construct without binding site). The negative control equals to 1.]] | ||
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+ | [[Image:MiMeasure_miR122.jpg|thumb|500px|left|''' different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid''']] | ||
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+ | [[Image:MiMeasure_miR122b.jpg|thumb|500px|left|''' different binding sites for miR122, Huh7 cells''']] | ||
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=====Flow cytometry measurements===== | =====Flow cytometry measurements===== | ||
[[Image:Flow1.jpg|thumb|600px|center|'''GFP/BFP ratio of single cells according to flow cytometry analysis''']] | [[Image:Flow1.jpg|thumb|600px|center|'''GFP/BFP ratio of single cells according to flow cytometry analysis''']] | ||
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===Analysis of miRaPCR Generated Binding Sites Against a Natural miRNA=== | ===Analysis of miRaPCR Generated Binding Sites Against a Natural miRNA=== |
Revision as of 13:12, 27 October 2010
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