Team:Heidelberg/Project/miMeasure
From 2010.igem.org
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The percentage of knockdown of each modified binding site can be determined by the comparison of the ratio of EGFP to EBFP2 ratio compared to the ratio of the perfect binding site caused knock-down. The ratio is derived from a linear regression curve. Therefore the knock-down efficiency can be conducted by various basic methods e.g. plate reading, flow cytometry or microscopy. | The percentage of knockdown of each modified binding site can be determined by the comparison of the ratio of EGFP to EBFP2 ratio compared to the ratio of the perfect binding site caused knock-down. The ratio is derived from a linear regression curve. Therefore the knock-down efficiency can be conducted by various basic methods e.g. plate reading, flow cytometry or microscopy. | ||
- | [[Image:MiMeasure.png]] | + | [[Image:MiMeasure.png|400px|Fig. 1: miMeasure plasmid.]] |
==Introduction== | ==Introduction== |
Revision as of 18:53, 26 October 2010
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