Team:Heidelberg/Parts/Characterization
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- | [[Image:Promoter_test_220910_hd2010.jpg| thumb | | + | [[Image:Promoter_test_220910_hd2010.jpg| thumb | 700px | centre | Promoter strength characterization of tuning constructs in HEK 293 T-REx cell line]] |
==Characterization of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K337035 BBa_K337035] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K337036 BBa_K337036] in different cell lines== | ==Characterization of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K337035 BBa_K337035] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K337036 BBa_K337036] in different cell lines== |
Revision as of 03:19, 27 October 2010
CharacterizationCharacterization of promoters in tuning constructs in T-Rex cellsDual Luciferase Assay
Characterization of BBa_K337035 and BBa_K337036 in different cell linesIf transfecting BBa_K337035 and BBa_K337036 are transfected into different cell lines it is obvious that Hek293T cells are the easiest to transfect with both constructs an expression of 17-22 RLU is to be measured. Hek T-Rex cells are showing and expression level of 12 RLU of both constructs. Hela cells are also showing constant expression levels of 8 RLU with both constructs. A rather low expression of 2RLU is to be seen by transfecting the 2 constructs in Huh7 cells. This might be due to low transfection efficiency of this cell line in general. All together it is to say that BBa_K337035 and BBa_K337036 show comparable expression.
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