Team:Heidelberg/Parts

From 2010.igem.org

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|F17||Luc2||[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]|| compatible for insertion of microRNA binding sites
|F17||Luc2||[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]|| compatible for insertion of microRNA binding sites
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|biCMV||biCMV||[http://partsregistry.org/Part:BBa_K337030 BBa_K337017]||-
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|F6||luc2_sv40ter fw||[http://partsregistry.org/Part:BBa_K337015 BBa_K337015]|| Luc2 reporter gene with terminator referring to SV40 promoter  
|F6||luc2_sv40ter fw||[http://partsregistry.org/Part:BBa_K337015 BBa_K337015]|| Luc2 reporter gene with terminator referring to SV40 promoter  
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|F15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression
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</center>
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== final constructs ==
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<center>
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{| class="wikitable sortable" border="0" style="text-align: left"
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|-bgcolor=#cccccc
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|+ align="top, left"|'''table 3''': Our final constructs. Listed ID refers to internal nomenclature.
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|width=40px|  ID||width=300px|Content||width=100px|Registry link||width=450px|Purpose of the cassette
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|K1||pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv40/CMV/Kozag_hRluc_BGH )||[http://partsregistry.org/Part:BBa_K337032 BBa_K337032]|| gene expression tuning
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|F15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression
|F15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression

Revision as of 14:08, 26 October 2010


Parts

We assembled a lot of miBricks (i. e. basic parts) to composite parts that were then used to built up our final constructs. The submitted parts are listed below. We did not categorize the parts according to different subprojects since they are universally applicable or clearly denoted through their names. Some binding site patterns, which were created via [... miraPCR], are listed separately.

basic parts

table 1: Our miBricks. Listed ID refers to internal nomenclature.
IDContentRegistry linkComment
F1RSV fwBBa_K337012-
F2SV40 rcBBa_K337013-
F3RSV rcBBa_K337014-
F4BGH fwBBa_K337001-
F5BGH rcBBa_K337002 purpose: insertion of BamHI sites
F7microRNA 10HDBBa_K337016 template for creation of shRNA-like miRNA
F8CMV fwBBa_K337018-
F9FRT siteBBa_K337019-
F10hRlucBBa_K337025-
F16TetRBBa_K337028-
F17Luc2BBa_K337030 compatible for insertion of microRNA binding sites
biCMVbiCMVBBa_K337017-

composite parts

table 2: Our composite parts from subsequent assembling steps. Listed ID refers to internal nomenclature.
IDContentRegistry linkPurpose of the cassette
F6luc2_sv40ter fwBBa_K337015 Luc2 reporter gene with terminator referring to SV40 promoter
F15CMV_TetO2BBa_K337027 CMV promoter under control of Tet Operator for regulation of gene expression

final constructs

table 3: Our final constructs. Listed ID refers to internal nomenclature.
IDContentRegistry linkPurpose of the cassette
K1pSMB_miTuner Plasmid HD1 (BGH(rc)/shRNA10(rc)/sv40(rc)/RSV/Luc2_sv40/CMV/Kozag_hRluc_BGH )BBa_K337032 gene expression tuning
F15CMV_TetO2BBa_K337027 CMV promoter under control of Tet Operator for regulation of gene expression

binding site patterns

table 4: Binding site patterns generated by miraPCR.
IDContentRegistry link
1.3hsa-miR-122 Binding site pattern (3BS)BBa_K337000
1Ahsa-miR-122 Binding site pattern (4BS)BBa_K337003
1-5hsa-miR-122 Binding site pattern (2BS)BBa_K337004
3.7hsa-miR-122 Binding site pattern (2BS - additional 10bp Spacer)BBa_K337005
1.5hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337006
1.8hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337007
3.1hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337008
4.5hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337009
4.6hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337010
mir221-10L2hsa-miR-221 Binding site pattern (2BS)BBa_K337010

<groupparts>iGEM010 Heidelberg</groupparts>



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