Team:Heidelberg/Parts

From 2010.igem.org

(Difference between revisions)
Line 49: Line 49:
==Parts==
==Parts==
-
We assembled a lot of '''miBricks''' (i. e. basic parts) to composite parts that were then used to built up our final constructs. The submitted parts are listed below. We did not categorize the parts according to different subprojects since they are universally applicable or clearly denoted through their names.
+
We assembled a lot of '''miBricks''' (i. e. basic parts) to composite parts that were then used to built up our final constructs. The submitted parts are listed below. We did not categorize the parts according to different subprojects since they are universally applicable or clearly denoted through their names. Some binding site patterns, which were created via [... miraPCR], are listed separately.  
<br/>
<br/>
Line 67: Line 67:
|F4||BGH fw||[http://partsregistry.org/Part:BBa_K337001 BBa_K337001]||-
|F4||BGH fw||[http://partsregistry.org/Part:BBa_K337001 BBa_K337001]||-
|-
|-
-
|F5||BGH rc||[http://partsregistry.org/Part:BBa_K337002 BBa_K337002]|| for insertion of BamHI sites
+
|F5||BGH rc||[http://partsregistry.org/Part:BBa_K337002 BBa_K337002]|| purpose: insertion of BamHI sites
|-
|-
-
|F7||microRNA 10HD||[http://partsregistry.org/Part:BBa_K337016 BBa_K337016]||-
+
|F7||microRNA 10HD||[http://partsregistry.org/Part:BBa_K337016 BBa_K337016]|| template for creation of shRNA-like miRNA
|-
|-
|F8||CMV fw||[http://partsregistry.org/Part:BBa_K337018 BBa_K337018]||-
|F8||CMV fw||[http://partsregistry.org/Part:BBa_K337018 BBa_K337018]||-
Line 79: Line 79:
|F16||TetR||[http://partsregistry.org/Part:BBa_K337028 BBa_K337028]||-
|F16||TetR||[http://partsregistry.org/Part:BBa_K337028 BBa_K337028]||-
|-
|-
-
|F17||Luc2*||[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]|| compatible for insertion of microRNA binding sites
+
|F17||Luc2||[http://partsregistry.org/Part:BBa_K337030 BBa_K337030]|| compatible for insertion of microRNA binding sites
|-
|-
|}
|}
Line 94: Line 94:
|-
|-
|F15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression
|F15||CMV_TetO2||[http://partsregistry.org/Part:BBa_K337027 BBa_K337027]|| CMV promoter under control of Tet Operator for regulation of gene expression
 +
|-
 +
|}
 +
</center>
 +
 +
=== binding site patterns ===
 +
<center>
 +
{| class="wikitable sortable" border="0" style="text-align: left"
 +
|-bgcolor=#cccccc
 +
|+ align="top, left"|'''table 4''': Binding site patterns generated by miraPCR.
 +
|width=40px|  ID||width=300px|Content||width=100px|Registry link
 +
|-
 +
|1.3||hsa-miR-122 Binding site pattern (3BS)||[http://partsregistry.org/Part:BBa_K337000 BBa_K337000]
 +
|-
 +
|1A||hsa-miR-122 Binding site pattern (4BS)||[http://partsregistry.org/Part:BBa_K337003 BBa_K337003]
 +
|-
 +
|1-5||hsa-miR-122 Binding site pattern (2BS)||[http://partsregistry.org/Part:BBa_K337004 BBa_K337004]
 +
|-
 +
|3.7||hsa-miR-122 Binding site pattern (2BS - additional 10bp Spacer)||[http://partsregistry.org/Part:BBa_K337005 BBa_K337005]
 +
|-
 +
|1.5||hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12||[http://partsregistry.org/Part:BBa_K337006 BBa_K337006]
 +
|-
 +
|1.8||hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12||[http://partsregistry.org/Part:BBa_K337007 BBa_K337007]
 +
|-
 +
|3.1||hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12||[http://partsregistry.org/Part:BBa_K337008 BBa_K337008]
 +
|-
 +
|4.5||hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12||[http://partsregistry.org/Part:BBa_K337009 BBa_K337009]
 +
|-
 +
|4.6||hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12||[http://partsregistry.org/Part:BBa_K337010 BBa_K337010]
 +
|-
 +
|mir221-10L2||hsa-miR-221 Binding site pattern (2BS)||[http://partsregistry.org/Part:BBa_K337010 BBa_K337010]
|-
|-
|}
|}

Revision as of 12:47, 26 October 2010


Contents

Parts

We assembled a lot of miBricks (i. e. basic parts) to composite parts that were then used to built up our final constructs. The submitted parts are listed below. We did not categorize the parts according to different subprojects since they are universally applicable or clearly denoted through their names. Some binding site patterns, which were created via [... miraPCR], are listed separately.

basic parts

table 1: Our miBricks.
Listed ID refers to internal nomenclature.
IDContentRegistry linkComment
F1RSV fwBBa_K337012-
F2SV40 rcBBa_K337013-
F3RSV rcBBa_K337014-
F4BGH fwBBa_K337001-
F5BGH rcBBa_K337002 purpose: insertion of BamHI sites
F7microRNA 10HDBBa_K337016 template for creation of shRNA-like miRNA
F8CMV fwBBa_K337018-
F9FRT siteBBa_K337019-
F10hRlucBBa_K337025-
F16TetRBBa_K337028-
F17Luc2BBa_K337030 compatible for insertion of microRNA binding sites

composite parts

table 2: Our composite parts from subsequent assembling steps. Listed ID refers to internal nomenclature.
IDContentRegistry linkPurpose of the cassette
F6luc2_sv40ter fwBBa_K337015 Luc2 reporter gene with terminator referring to SV40 promoter
F15CMV_TetO2BBa_K337027 CMV promoter under control of Tet Operator for regulation of gene expression

binding site patterns

table 4: Binding site patterns generated by miraPCR.
IDContentRegistry link
1.3hsa-miR-122 Binding site pattern (3BS)BBa_K337000
1Ahsa-miR-122 Binding site pattern (4BS)BBa_K337003
1-5hsa-miR-122 Binding site pattern (2BS)BBa_K337004
3.7hsa-miR-122 Binding site pattern (2BS - additional 10bp Spacer)BBa_K337005
1.5hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337006
1.8hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337007
3.1hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337008
4.5hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337009
4.6hsa-miR-122 Binding site pattern (2BS) with randomized nt9-12BBa_K337010
mir221-10L2hsa-miR-221 Binding site pattern (2BS)BBa_K337010

<groupparts>iGEM010 Heidelberg</groupparts>



Retrieved from "http://2010.igem.org/Team:Heidelberg/Parts"