Team:Heidelberg/Notebook/miRNA Kit/September
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* a lot of religations, thus: a lot of colonies to pick | * a lot of religations, thus: a lot of colonies to pick | ||
* [[Igem2010/Main/Protocols/Colony_PCR | colony-PCR]] and test digestion with SpeI and PstI of promising mini-preps revealed some positive samples | * [[Igem2010/Main/Protocols/Colony_PCR | colony-PCR]] and test digestion with SpeI and PstI of promising mini-preps revealed some positive samples | ||
- | * ligation with [[Igem2010/Main/Library/Constructs/pSMB miTrigger#basal components and cloning strategy | R8]] and [[Igem2010/Main/Library/Constructs/pSMB miTrigger#basal components and cloning strategy | R11]], respectively (each digested with EcoRI and NheI) in | + | * ligation with [[Igem2010/Main/Library/Constructs/pSMB miTrigger#basal components and cloning strategy | R8]] and [[Igem2010/Main/Library/Constructs/pSMB miTrigger#basal components and cloning strategy | R11]], respectively (each digested with EcoRI and NheI) in [http://partsregistry.org/Part:pSB1C3 pSB1C3] (backbone, digested with EcoRI and PstI), following [https://2010.igem.org/3A_Assembly standard protocol recommendations] |
* growth of transformed cells on selective agar plates (chloramphenicol) over night | * growth of transformed cells on selective agar plates (chloramphenicol) over night | ||
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Revision as of 20:59, 26 October 2010
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