Team:Heidelberg/Notebook/miRNA Kit/September
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- | * digestion of [[Igem2010/Main/Library/Constructs/pSMB miTrigger#basal components and cloning strategy | R23]] with EcoRI and PstI in EcoRI Buffer ([http://www.neb.com NEB]) and ligation into digested | + | * digestion of [[Igem2010/Main/Library/Constructs/pSMB miTrigger#basal components and cloning strategy | R23]] with EcoRI and PstI in EcoRI Buffer ([http://www.neb.com NEB]) and ligation into digested [http://partsregistry.org/Part:pSB1A3 pSB1A3], SAP of [http://partsregistry.org/Part:pSB1A3 pSB1A3], transformation, growing on selective agar plates (chloramphenicol), following [https://2010.igem.org/3A_Assembly standard protocol recommendations] |
Revision as of 20:50, 26 October 2010
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