Team:Heidelberg/Notebook/miRNA Kit

From 2010.igem.org

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= Synthetic miRNA Kit =
= Synthetic miRNA Kit =
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[[Image:MiTuner p.png|400px| miTuner plasmid]]
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This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNA-like miRNAs and referring binding sites using an oligo and PCR based system.
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You do not know where to start? Why don't you [http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner design your binding sites] first!
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After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the [http://2010.igem.org/Team:Heidelberg/Notebook/miMeasure mi Measure notebook] and see miMeasure in action on our [http://2010.igem.org/Team:Heidelberg/Project/miMeasure measurements] page!
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Now you would like to deliver you vector to a patient? Why not use a very specific [http://2010.igem.org/Team:Heidelberg/Project/Capsid_Shuffling/ViroBytes shuttle]?
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Latest revision as of 00:35, 28 October 2010

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Synthetic miRNA Kit

miTuner plasmid

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