Team:Heidelberg/Notebook/miRNA Kit

From 2010.igem.org

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= Synthetic miRNA Kit =
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This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNA-like miRNAs and referring binding sites using an oligo and PCR based system.
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You do not know where to start? Why don't you [http://2010.igem.org/Team:Heidelberg/Modeling/miBSdesigner design your binding sites] first!
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After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the [http://2010.igem.org/Team:Heidelberg/Notebook/miMeasure mi Measure notebook] and see miMeasure in action on our [http://2010.igem.org/Team:Heidelberg/Project/miMeasure measurements] page!
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Now you would like to deliver you vector to a patient? Why not use a very specific [http://2010.igem.org/Team:Heidelberg/Project/Capsid_Shuffling/ViroBytes shuttle]?
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{{:Team:Heidelberg/Pagemiddle}}
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{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#009be1; border: 1.5px solid #000000;"
{| cellpadding="5" cellspacing="0" align="center" style="text-align: center; color:#009be1; border: 1.5px solid #000000;"
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|width="20pt"|'''M'''||width="20pt"|'''T'''||width="20pt"|'''W'''||width="20pt"|'''T'''||width="20pt"|'''F'''||width="20pt"|'''S'''||width="20pt"|'''S'''
|- style="background:#f2f2f2; color:#78b41e"
|- style="background:#f2f2f2; color:#78b41e"
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|colspan="4"| ||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#01.2F09.2F2010 1]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#02.2F09.2F2010 2]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#03.2F09.2F2010 3]'''
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|colspan="4"| ||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#01.2F10.2F2010 1]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#02.2F10.2F2010 2]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#03.2F10.2F2010 3]'''
|- style="background:#f2f2f2; color:#f09600"
|- style="background:#f2f2f2; color:#f09600"
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#04.2F09.2F2010 4]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#05.2F09.2F2010 5]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#04.2F10.2F2010 4]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#05.2F10.2F2010 5]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#06.2F09.2F2010 6]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#07.2F09.2F2010 7]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#08.2F09.2F2010 8]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#09.2F09.2F2010 9]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#10.2F09.2F2010 10]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#06.2F10.2F2010 6]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#07.2F10.2F2010 7]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#08.2F10.2F2010 8]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#09.2F10.2F2010 9]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#10.2F10.2F2010 10]'''
|- style="background:#f2f2f2; color:#f09600"
|- style="background:#f2f2f2; color:#f09600"
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#11.2F09.2F2010 11]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#12.2F09.2F2010 12]'''|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#13.2F09.2F2010 13]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#14.2F09.2F2010 14]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#15.2F09.2F2010 15]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#16.2F09.2F2010 16]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#17.2F09.2F2010 17]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#18.2F09.2F2010 18]'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#11.2F10.2F2010 11]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#12.2F10.2F2010 12]'''|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#13.2F10.2F2010 13]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#14.2F10.2F2010 14]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#15.2F09.2F2010 15]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#16.2F10.2F2010 16]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#17.2F10.2F2010 17]'''||'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#18.2F10.2F2010 18]'''
|- style="background:#f2f2f2; color:#f09600"
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#19.2F09.2F2010 19]'''||'''20'''||'''21'''||'''22'''||'''23'''||'''24'''||'''25'''
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|'''[http://2010.igem.org/Team:Heidelberg/Notebook/miRNA_Kit/October#19/10/2010 19]'''||'''20'''||'''21'''||'''22'''||'''23'''||'''24'''||'''25'''
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= Synthetic miRNA Kit =
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<center>
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[[Image:MiTuner p.png|400px| miTuner plasmid]]
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>/center>
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{{:Team:Heidelberg/Single_Bottom}}

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Synthetic miRNA Kit

miTuner plasmid

>/center>