Team:Heidelberg/Notebook/miRNA Kit

From 2010.igem.org

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Revision as of 15:40, 26 October 2010

Synthetic miRNA Kit

This Kit has everything you need to construct a personalized gene expression vector controlled by synthetic miRNAs and miRNA binding sites. In our notebook, you will find out how to create shRNA-like miRNAs and referring binding sites using an oligo and PCR based system.

You do not know where to start? Why don't you design your binding sites first!

After construction of your binding sites, you want to test their knockdown efficiency? No problem! Just clone them into miMeasure, our measurement standard! Find out how to in the mi Measure notebook and see miMeasure in action on our measurements page!

Now you would like to deliver you vector to a patient? Why not use a very specific shuttle?



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