Team:Heidelberg/Notebook/miMeasure

From 2010.igem.org

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miMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency does not affect result we decided to include reference fluorescence protein, BFP to allow normalisation.
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miMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency or amount of cells do not affect result we decided to include reference fluorescence protein, BFP to allow normalisation.
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[[Image:MiMeasure.png|500px|miMeasure plasmid]]
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[[Image:MiMeasure.png|500px|left|miMeasure plasmid]]
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Latest revision as of 03:53, 28 October 2010

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miMeasure

miMeasure allows to quantitatively detect endogenous miRNA in different cells. To detect miRNA one should clone binding site for it into 3' UTR of GFP gene. To ensure that transfection efficiency or amount of cells do not affect result we decided to include reference fluorescence protein, BFP to allow normalisation.


miMeasure plasmid