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Team:Heidelberg/Notebook/miMeasure
From 2010.igem.org
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| - | ==Seeding and transfection of cells for TECAN, flow cytometry, plate reader and microscopy== | + | ===Seeding and transfection of cells for TECAN, flow cytometry, plate reader and microscopy=== |
5000 cells were seeded on day one in each well of the 96 well plate. Transfection of the constructs (M12-M22) with four different conditions were carried out on day two. The ratio of transfection is 1 (M construct) : 5 (stuffer/ miRsAg/ pcDNA5/ shRNA3) with a total amount | 5000 cells were seeded on day one in each well of the 96 well plate. Transfection of the constructs (M12-M22) with four different conditions were carried out on day two. The ratio of transfection is 1 (M construct) : 5 (stuffer/ miRsAg/ pcDNA5/ shRNA3) with a total amount | ||
Revision as of 18:57, 24 October 2010
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