Team:Heidelberg/Notebook/MiMeasure/October

From 2010.igem.org

(Difference between revisions)
(26/10/2010)
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[[Image:MiMeasure_miR122.jpg‎‎|thumb|500px|left|''' different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid''']]
[[Image:MiMeasure_miR122.jpg‎‎|thumb|500px|left|''' different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid''']]
 +
[[Image:MiMeasure_miR122b.jpg‎‎|thumb|500px|left|''' different binding sites for miR122, Huh7 cells''']]
[[Image:MiMeasure_miR122b.jpg‎‎|thumb|500px|left|''' different binding sites for miR122, Huh7 cells''']]
Flow cytometry measurements
Flow cytometry measurements
[[Image:Flow.jpg|thumb|500px|left|'''GFP/BFP ratio of single cells according to flow cytometry analysis''']]
[[Image:Flow.jpg|thumb|500px|left|'''GFP/BFP ratio of single cells according to flow cytometry analysis''']]
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{{:Team:Heidelberg/Single_Bottom}}
{{:Team:Heidelberg/Single_Bottom}}

Revision as of 13:11, 27 October 2010

July
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August
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9101112131415
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3031


September
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2728293031

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October
MTWTFSS
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45 678910
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2627282930

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21/10/2010

seeding HeLa and Huh7 in 96-well plates

22/10/2010

Transfection

23/10/2010

seeding HeLa and Huh7 in 96-well plates

24/10/2010

HeLa cells were transfected with miMeasure constructs with GFP expression regulated by different imperfect binding sites for miRsAg. As controls we used miMeasure construct with perfect binding site and no binding site. Each miMeasure was cotransfected with pcDNA plasmid expressing synthetic miRsAg and pcDNA5 expressing synthetic shRNA3.


Transfection of HeLa and Huh7 cells with miMeasure with binding sites for miR122.

Huh7 were transfected with 50ng of miMeasure. HeLa cells were used as a control they were transfected with miMeasure constructs and miR122. Control without miR122 expression plasmid was made.

26/10/2010

microscopy measurements

different binding sites for miRsAg
different binding sites for miR122, HeLa cotransfected with miR122 expression plasmid
different binding sites for miR122, Huh7 cells

Flow cytometry measurements

GFP/BFP ratio of single cells according to flow cytometry analysis