Team:Heidelberg/Notebook/Homology Based/August

From 2010.igem.org

(Difference between revisions)
Line 75: Line 75:
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#19.2F09.2F2010 19]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#20.2F09.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#21.2F09.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#22.2F09.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#23.2F09.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#24.2F09.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#25.2F09.2F2010 25]'''
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#19.2F09.2F2010 19]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#20.2F09.2F2010 20]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#21.2F09.2F2010 21]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#22.2F09.2F2010 22]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#23.2F09.2F2010 23]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#24.2F09.2F2010 24]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#25.2F09.2F2010 25]'''
|- style="background:#f2f2f2; color:#78b41e"
|- style="background:#f2f2f2; color:#78b41e"
-
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#26.2F09.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#27.2F09.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#28.2F09.2F2010 28]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#29.2F09.2F2010 29]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#22.2F30.2F2010 30]'''||colspan="3"|
+
|'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#26.2F09.2F2010 26]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#27.2F09.2F2010 27]'''||'''[https://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/October#28.2F09.2F2010 28]'''||'''29'''||'''30'''||colspan="3"|
|-  style="background:#f2f2f2; color:#78b41e"
|-  style="background:#f2f2f2; color:#78b41e"
| colspan="7"| <span style="color:#ffffff">-</span>
| colspan="7"| <span style="color:#ffffff">-</span>

Revision as of 16:31, 26 October 2010

Homology Based Capsid Shuffling

31/08/2010

  • we decided to use AAV serotypes 1,2,5,6,8 and 9 as those have been described in the literature to infect pancreas cells.
  • cap plasmids (AAV 1, 2, 5, 6, 8, 9) have been diluted to a concentration of 200ng/ul
  • PCR on cap genes was performed using M13 primers as the genes are in the pBluescript vector:
    • AAV1 A
    • AAV2 A & B
    • AAV5 A & B
    • AAV6
    • AAV8 A & B
    • AAV9 A


August
MTWTFSS
1
2345678
9101112131415
16171819202122
23242526272829
30


September
MTWTFSS
12345
6789101112
13141516171819
20212223242526
27282930
-


October
MTWTFSS
123
45 678910
11131415161718
19202122232425
2627282930
-


Retrieved from "http://2010.igem.org/Team:Heidelberg/Notebook/Homology_Based/August"