Revision as of 12:49, 25 October 2010 by AlejandroHD (Talk | contribs)

BS designer for miRNA

Construction of microRNA binding sites
Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8)
Customized MISMATCH position

Supplementary region
Customized MATCH positions (input numbers from 9 to 22)

Modify AU content
A in position -1
A in position 0
A in position 1
A in position 8
A in position 9
A in position 10

Sticky ends for integration in plasmid:
Customized: 5' 3'

Name of primer 1
<textarea name="NamA" id="NamA" class="variaresult" wrap=SOFT></textarea>
Sequence of primer 1
<textarea name="seqA" id="seqA" class="variaresult" wrap=SOFT></textarea>
Name of primer 2
<textarea name="NamB" id="NamB" class="variaresult" wrap=SOFT></textarea>
Sequence of primer 2
<textarea name="seqB" id="seqB" class="variaresult" wrap=SOFT></textarea>