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Team:Harvard/flavor/flavors
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| - | <div> | + | <div>wintergreen expression system <a href="https://static.igem.org/mediawiki/2010/b/bd/Wintergreen_pathway.png" id="single_image" style="font-size:12px">click to enlarge</a></div><hr/> |
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| - | <p style="padding:10px"><br/>The | + | <p style="padding:10px"><br/>The MIT 2006 iGEM team created a BioBricked system to express <i>methyl salicyate</i>, a mint smelling compound. By swapping-in plant specific promoters and inserting the system into the pORE open vector, we would be able to create mint-smelling plants. </p></td> |
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Revision as of 16:01, 10 October 2010
Flavors
Miraculin
Miraculin is 'flavor inverting' protein, found naturally in the fruit of the plant fruit of Synsepalum dulcificum. Not sweet by itself, miraculin binds to taste receptors on the tongue, possibly altering the structure of the receptors and causing traditionally 'sour' flavors to be received as 'sweet'.
We have obtained, synthesized and BioBricked the DNA sequence of miraculin. This has allowed us to work with it in the usual BioBrick fashion, facilitating insertion into the agrobacterium expression vector.
Preliminary tests in E. Coli have been positive, with our YFP-tagged proteins showing definite expression
Brazzein
Valencia
Wintergreen
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wintergreen expression system click to enlarge 
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