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Team:Harvard/color/promoters
From 2010.igem.org
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<h1>targeting the petals</h1> | <h1>targeting the petals</h1> | ||
| - | <p>In addition to using a constitutive promoter to express our knockdown constructs, we will also express these constructs in parallel with petal specific promoters. Using <a href="http://www.genevestigator.com/">Genevestigator's</a> Biomarker Search tool, we identified genes with high petal expression. We then looked up those genes <a href="http://aranet.mpimp-golm.mpg.de/aranet/Home">AraGenNet</a>for more specific information about expression, and from those that looked the most promising, found promoter information on the Plant Promoter Database by Yoshiharu Y Yamamoto and Junichi Obokata at the Center for Gene Research at Nagoya University (<a href="http://ppdb.gene.nagoya-u.ac.jp>ppdb.gene.nagoya-u.ac.jp</a>). From this database, we selected sequences from 1 to 1.5 kb upstream from the genes to PCR amplify from arabidopsis genomic DNA.</p> | + | <p>In addition to using a constitutive promoter to express our knockdown constructs, we will also express these constructs in parallel with petal specific promoters. Using <a href="http://www.genevestigator.com/">Genevestigator's</a> Biomarker Search tool, we identified genes with high petal expression. We then looked up those genes <a href="http://aranet.mpimp-golm.mpg.de/aranet/Home">AraGenNet</a>for more specific information about expression, and from those that looked the most promising, found promoter information on the Plant Promoter Database by Yoshiharu Y Yamamoto and Junichi Obokata at the Center for Gene Research at Nagoya University (<a href="http://ppdb.gene.nagoya-u.ac.jp">ppdb.gene.nagoya-u.ac.jp</a>). From this database, we selected sequences from 1 to 1.5 kb upstream from the genes to PCR amplify from arabidopsis genomic DNA.</p> |
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<h1>the genes</h1> | <h1>the genes</h1> | ||
Revision as of 14:39, 30 July 2010
abstract lab notebook pigment metabolism engineering pathways targeting the petals parts & primers results references
targeting the petals
In addition to using a constitutive promoter to express our knockdown constructs, we will also express these constructs in parallel with petal specific promoters. Using Genevestigator's Biomarker Search tool, we identified genes with high petal expression. We then looked up those genes AraGenNetfor more specific information about expression, and from those that looked the most promising, found promoter information on the Plant Promoter Database by Yoshiharu Y Yamamoto and Junichi Obokata at the Center for Gene Research at Nagoya University (ppdb.gene.nagoya-u.ac.jp). From this database, we selected sequences from 1 to 1.5 kb upstream from the genes to PCR amplify from arabidopsis genomic DNA.
