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Team:Harvard/color/inhibition
From 2010.igem.org
abstract lab notebook pigment metabolism engineering pathways targeting the petals parts & primers results references
the plan
As stated in the abstract, our goal is to accumulate the pigments lycopene and beta-carotene in the petals of arabidopsis, and we plan to do so by inhibiting the enzymes lycopene epsilon cyclase (LUT2), carotene beta-ring hydroxylase (BETA-OHASE 1), and lycopene beta cyclase (LYC) via artificial microRNA interference.
In our first scheme, we will knock out LUT2 on its own. We hope that this will induce accumulation of lycopene.
