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Team:Edinburgh/Notebook/Blue light sensor
From 2010.igem.org
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== Blue Light Sensor == | == Blue Light Sensor == | ||
| + | <br><br> | ||
| + | <b>04/10/10</b> | ||
<br> | <br> | ||
| + | We may have a working LovTap construct + red/white (RFP) reporter system thanks to Mexico. | ||
| + | <br> | ||
| + | Minor characterisation experiment:<br> | ||
| + | -LovTap constructed with red-white RFP reporter<br> | ||
| + | -If activated, the blue light sensor transformants should produce red colonies<br> | ||
| + | -Blue light sensor containing-colonies from transformant plate streaked onto two plates<br> | ||
| + | -One plate left in the light, the other left in the dark<br> | ||
| + | -If the one in the light grows red and the one in the dark doesn't, it is a good indication that the construct is working<br><br> | ||
Revision as of 11:59, 4 October 2010
Blue Light Sensor
04/10/10
We may have a working LovTap construct + red/white (RFP) reporter system thanks to Mexico.
Minor characterisation experiment:
-LovTap constructed with red-white RFP reporter
-If activated, the blue light sensor transformants should produce red colonies
-Blue light sensor containing-colonies from transformant plate streaked onto two plates
-One plate left in the light, the other left in the dark
-If the one in the light grows red and the one in the dark doesn't, it is a good indication that the construct is working
