Team:Edinburgh

From 2010.igem.org

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<ul class="dropdown dropdown-horizontal">
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  <li style="width:6%;"><a href="https://2010.igem.org/Team:Edinburgh" class="dir">home</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh" class="dir">home</a>
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  <li style="width:13%;"><a href="https://2010.igem.org/Team:Edinburgh/Team" class="dir">team - illuminati</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Team" class="dir">team - illuminati</a>
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   <ul>
   <li><a href="https://igem.org/Team.cgi?id=322">official</a></li>
   <li><a href="https://igem.org/Team.cgi?id=322">official</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Students">students</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Students">students</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Environment">environment</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Environment">environment</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Gallery" class="dir">gallery</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Gallery" class="dir">gallery</a></li>
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  <li style="width:15%;"><a href="https://2010.igem.org/Team:Edinburgh/Project" class="dir">genomic BRIDGEs</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Project" class="dir">genomic BRIDGEs</a>
   <ul>
   <ul>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">the protocol</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Protocol">the protocol</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">biobrick parts</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Genomic">submitted parts</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">results</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Genomic">results</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">future work</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/Future">the future</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">references</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Project/References">references</a></li>
   </ul>
   </ul>
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  </li>
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  <li style="width:15%;"><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
   <ul>
   <ul>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the repressilator</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the project</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red light</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_sensor">red sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Red_light_producer">red producer</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Blue_light_producer">blue light</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Blue_light_sensor">blue sensor</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Blue_light_sensor">blue sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Blue_light_producer">blue producer</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_producer">green light</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_sensor">green sensor</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_sensor">green sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Green_light_producer">green producer</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/BioBricks#Bacterial">submitted parts</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial">biobrick parts</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Bacterial">results</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial">results</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Future">the future</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial">future work</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/References">references</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial">references</a></li>
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   </ul>
   </ul>
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  </li>
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  <li style="width:15%;"><a href="https://2010.igem.org/Team:Edinburgh/Modelling" class="dir">modelling BRIDGEs</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling" class="dir">modelling BRIDGEs</a>
   <ul>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling">the model</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Kappa">kappa</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling">results</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Genomic">the genomic model</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling">future work</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Bacterial">the bacterial model</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling">references</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Signalling">the signalling model</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Tools">tools</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Modelling">results</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/Future">the future</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling/References">references</a></li>
   </ul>
   </ul>
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  <li style="width:14%;"><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
   <ul>
   <ul>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human">human aspects</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Communication">communication of science</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human">results</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Branding">iGEM survey</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human">future work</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Conversations">conversations</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Human">references</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/FutureApps">future applications</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">further thoughts</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li>
   </ul>
   </ul>
  </li>
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  <li style="width:10%;"><a href="https://2010.igem.org/Team:Edinburgh/Notebook" class="dir">lab notes&nbsp;&nbsp;&nbsp;</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook" class="dir">lab notes&nbsp;&nbsp;&nbsp;</a>
   <ul>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">collaboration</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Collaboration">collaboration</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">BRIDGE</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Attribution">attribution</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">red light</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/BRIDGE">BRIDGE</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">red sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_producer">red light</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">blue light</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_sensor">red sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">blue sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_producer">blue light</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">safety</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_sensor">blue sensor</a></li>
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   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Green_light_producer">green light</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Green_light_sensor">green sensor</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Modelling">modelling</a></li>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Safety">safety</a></li>
   <li><a href="http://www.openwetware.org/wiki/French_Lab">protocols</a></li>
   <li><a href="http://www.openwetware.org/wiki/French_Lab">protocols</a></li>
   </ul>
   </ul>
  </li>
  </li>
   
   
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  <li style="width:12%;"><a href="https://2010.igem.org/Team:Edinburgh/Acknowledgements" class="dir">acknowledgements</a>
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  <li><a href="https://2010.igem.org/Team:Edinburgh/Acknowledgements" class="dir">acknowledgements</a>
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<a href="https://2010.igem.org/Team:Edinburgh/Team"><img src="https://static.igem.org/mediawiki/2010/0/0d/Ed10-Illuminati.jpg"></a><br><br>
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<a href="https://2010.igem.org/Team:Edinburgh/Team"><img src="https://static.igem.org/mediawiki/2010/0/06/Ed10-Team-illuminati.png"><br></a>
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<img src="https://static.igem.org/mediawiki/2010/8/8c/Ed10-The_project.png"></a><br><br>
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The 2010 University of Edinburgh iGEM team, known as the Illuminati, will approach the problem of building bridges in three distinct ways.
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<b>The 2010 <a class="headlink" href="http://www.ed.ac.uk/home">University of Edinburgh</a> iGEM <a class="headlink" href="https://2010.igem.org/Team:Edinburgh/Team">team</a> applied the concept of building bridges throughout our project in three distinct ways:
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<ul style="list-style-type: disc; list-style-image: none;">
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<li>A structured framework for non-chemical <a class="headlink" href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">communication</a> between <i>E. coli</i>, codenamed <a class="headlink" href="https://2010.igem.org/Team:Edinburgh/Bacterial">FORTH</a>, pairing light-producing and light-sensing BioBricks such that future projects can be developed for a variety of novel applications.
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</li>
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<li>The <a class="headlink" href="https://2010.igem.org/Team:Edinburgh/Project">BRIDGE</a> protocol, a markerless alternative for BioBrick insertion via homologous recombination.
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</li>
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<li>Examining synthetic biology as <a class="headlink" href="https://2010.igem.org/Team:Edinburgh/Human">ways of thinking</a> and the permeation of human practices within, bridging the so-called 'divides' between various disciplines and individuals.</b>
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</li>
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<a href="https://2010.igem.org/Team:Edinburgh/Project"><img src="https://static.igem.org/mediawiki/2010/3/3b/Ed10-Genomic.jpg"></a><br><br>
 
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<a href="https://2010.igem.org/Team:Edinburgh/Project"><img src="https://static.igem.org/mediawiki/2010/d/d6/Ed10-Genomic_BRIDGEs.png"><br></a>
 
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At a genomic level, BRIDGE is the name of a BioBrick-compatible construct used for two-step marker-less insertion and deletion of genes.
 
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<a href="https://2010.igem.org/Team:Edinburgh/Bacterial"><img src="https://static.igem.org/mediawiki/2010/8/8f/Ed10-Bacterial.jpg"></a><br><br>
 
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<a href="https://2010.igem.org/Team:Edinburgh/Bacterial"><img src="https://static.igem.org/mediawiki/2010/7/7f/Ed10-BacterialBRIDGEs.png"><br></a>
 
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At a bacterial level, bridges of light will allow bacteria to communicate and coordinate their actions.<br><br></td>
 
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<a href="https://2010.igem.org/Team:Edinburgh/Modelling"><img src="https://static.igem.org/mediawiki/2010/1/1b/Ed10-Modelling.jpg"></a><br><br>
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<a href="https://2010.igem.org/Team:Edinburgh/BioBricks"><img src="https://static.igem.org/mediawiki/2010/7/7c/Ed10-Submitted_BioBrickspng.png"></a>
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<a href="https://2010.igem.org/Team:Edinburgh/Modelling"><img src="https://static.igem.org/mediawiki/2010/8/8b/Ed10-ModellingBRIDGEs.png"><br></a>
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Modelling of the bacterial BRIDGEs will give us greater insight into and understanding of cellular mechanisms.<br><br>
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<center><img src="https://static.igem.org/mediawiki/2010/f/ff/Ed10-Bridge.png" height="120px"></center>
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<a href="https://2010.igem.org/Team:Edinburgh/Results"><img src="https://static.igem.org/mediawiki/2010/4/41/Ed10-Project_resultspng.png"></a>  
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<a href="https://2010.igem.org/Team:Edinburgh/Human"><img src="https://static.igem.org/mediawiki/2010/8/84/Ed10-Human.jpg"></a><br><br>
 
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<a href="https://2010.igem.org/Team:Edinburgh/Human"><img src="https://static.igem.org/mediawiki/2010/b/b8/Ed10-HumanBRIDGEs.png"><br></a>
 
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And at a human level, bridges of understanding will traverse gaps between individuals, disciplines, and ways of thinking.</td>
 
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<p><center><i>"The engineering equivalent of Genetic Engineering is to get a bunch of concrete and steel, throw it into a river,
 
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<br> and if you can walk across it, call it a bridge."</i>
 
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<br> - <a href="http://www.leagueagainsttedium.co.uk/">Simon Munnery</a>, comedian.</center></p>
 
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<img src="https://static.igem.org/mediawiki/2010/4/4f/Ed10-Bridging_through_iGEM.png"><br>
<img src="https://static.igem.org/mediawiki/2010/4/4f/Ed10-Bridging_through_iGEM.png"><br>
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Synthetic biology in general, and iGEM in particular, has long attempted to refine this process of "bridge-building". Among these efforts include the establishment of standards that can be applied across the discipline, as well as the development of standardised BioBricks and their collation in a public registry of parts. In this way, iGEM participants attempt to pave the way for future endeavours - the possibilities of building bridges instead of simply stumbling across them by chance, of using standardised bricks instead of having to quarry and hew individual stones, and of developing innovative new ways of creating bridges from scratch.</p>
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<p>"The engineering equivalent of Genetic Engineering is to get a bunch of concrete and steel, throw it into a river, and if you can walk across it, call it a bridge." - <a href="http://www.leagueagainsttedium.co.uk/">Simon Munnery</a>, comedian.</p>
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<p>The question is... how do you think?</p></td>
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<p>Synthetic biology in general, and <a href="https://2010.igem.org/Main_Page">iGEM</a> in particular, has long attempted to refine this process of <b>"bridge-building"</b>. iGEM <b>participants</b> attempt to pave the way for future <b>endeavours</b> - the possibilities of building bridges instead of simply stumbling across them by chance, of using standardised <a href="http://partsregistry.org/Main_Page">bricks</a> instead of having to quarry and hew individual stones, and of developing innovative new ways of <b>creating</b> bridges from scratch.</p>
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   <td width="50$" style="border-color:transparent;"><img src="https://static.igem.org/mediawiki/2010/9/96/Ed10-2bridges.jpg"></td>
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<p>We think about synthetic biology differently now. The question is... how do <b>you</b> think?</p></td>
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   <td width="50%" style="border-color:transparent;"><img src="https://static.igem.org/mediawiki/2010/9/96/Ed10-2bridges.jpg"></td>
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<p>The <b>Illuminati</b> were a secret <b>society</b> that existed in Bavaria in the late 18th century. They were known for their aims to <b>encourage enlightenment</b>, <b>education</b>, and the spread of <b>knowledge</b> via such radical <b>ideals</b> as <b>liberty</b> of <b>thought</b> and <b>equality</b> amongst <b>people</b>. Their short-lived movement did not succeed, but many of their tenets are <b>willingly</b> espoused today as fundamental <b>rights of freedom</b>.</p>
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<p>The <a href=https://2010.igem.org/Team:Edinburgh/Team">2010 University of Edinburgh iGEM team</a> has taken up their name in <b>honour</b> of their <b>efforts</b> to bridge the schisms that existed in <b>society</b> at their <b>time</b>. In the same way, we <b>hope</b> that <b>our</b> light may begin to bridge the divides that exist in synthetic biology today: <b>ideas</b> into reality, <b>communication</b> between bacteria, and <b>ways of thinking</b> amongst <b>disciplines</b> and <b>individuals</b>.</p>
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<span style="color:ivory;">Throughout this wiki there are words in <b>bold</b> that indicate a relevance to <a href="https://2010.igem.org/Team:Edinburgh/Human">human practices</a>. It will become obvious that <b>human practices</b> are a part of almost everything in <b>iGEM</b>.</span>
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Latest revision as of 01:58, 28 October 2010








The 2010 University of Edinburgh iGEM team applied the concept of building bridges throughout our project in three distinct ways:
  • A structured framework for non-chemical communication between E. coli, codenamed FORTH, pairing light-producing and light-sensing BioBricks such that future projects can be developed for a variety of novel applications.
  • The BRIDGE protocol, a markerless alternative for BioBrick insertion via homologous recombination.
  • Examining synthetic biology as ways of thinking and the permeation of human practices within, bridging the so-called 'divides' between various disciplines and individuals.


"The engineering equivalent of Genetic Engineering is to get a bunch of concrete and steel, throw it into a river, and if you can walk across it, call it a bridge." - Simon Munnery, comedian.

Synthetic biology in general, and iGEM in particular, has long attempted to refine this process of "bridge-building". iGEM participants attempt to pave the way for future endeavours - the possibilities of building bridges instead of simply stumbling across them by chance, of using standardised bricks instead of having to quarry and hew individual stones, and of developing innovative new ways of creating bridges from scratch.

We think about synthetic biology differently now. The question is... how do you think?

The Illuminati were a secret society that existed in Bavaria in the late 18th century. They were known for their aims to encourage enlightenment, education, and the spread of knowledge via such radical ideals as liberty of thought and equality amongst people. Their short-lived movement did not succeed, but many of their tenets are willingly espoused today as fundamental rights of freedom.

The 2010 University of Edinburgh iGEM team has taken up their name in honour of their efforts to bridge the schisms that existed in society at their time. In the same way, we hope that our light may begin to bridge the divides that exist in synthetic biology today: ideas into reality, communication between bacteria, and ways of thinking amongst disciplines and individuals.

Throughout this wiki there are words in bold that indicate a relevance to human practices. It will become obvious that human practices are a part of almost everything in iGEM.