Team:Chiba/lux promoter

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(Difference between revisions)

Revision as of 11:36, 27 October 2010

Lux promoter inverter

We've charaterized Plux inverter.
There is promoter (HSL-luxR repressor), biobrick number R0061.
We've prepare Plux inv-GFP and characterized about it.

R0061の下流にRBSおよびGFP遺伝子 (E0240) を挿入したプラスミド (Plux inv-GFP) 作製し,LuxRの抑制機能を調べた

Experiments

1.Preparing Plux inv-GFP

Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed.

Fig. 1 cloning process

2.Characterization Plux inv-GFP

Results

Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。

Fig. 2 assay process

Reference

1) Egland.K.A, and Greenberg.E.P, Conversion of the ''Vibrio Fischeri'' Transcriptional Activator,LuxR, to a Repressor, ''J. Bacteriol.'', '''182''', P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, ''Mol Syst Biol'', '''3''' ,145 (2007)

@@ Line 188: / Line 188: @@
 We've charaterized Plux inverter.<br>
 There is promoter (HSL-luxR repressor), biobrick number R0061.<br>
-We've made Plux inv-GFP and characterized about it.<br><br>
+We've prepare Plux inv-GFP and characterized about it.<br><br>
+R0061の下流にRBSおよびGFP遺伝子 (E0240) を挿入したプラスミド (Plux inv-GFP) 作製し,LuxRの抑制機能を調べた
+<br>
 </font></td>
 </tr>
@@ Line 202: / Line 205: @@
 <font size="3">1.Preparing Plux inv-GFP</font><br><br>
 &nbsp;&nbsp;
-cloning process is shown in Fig. 1
+Preparing Plux inv-GFP process is shown in '''Fig. 1'''.GFPの蛍光が確認されたので，Plux inv-GFPは機能している。 and sequence is conformed.
 </font></td>
 </tr>
@@ Line 219: / Line 222: @@
 <font size="3">
+Plux inv-GFP およびLuxR generatorである Plac-LuxRをDH10B株に共形質転換した。
 </font></td>