Team:Chiba/lux promoter

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[[Image:Plux inv-GFP.png|frame|center|Fig. 1 cloning process]]
[[Image:Plux inv-GFP.png|frame|center|Fig. 1 cloning process]]
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<font size="3">2.Characterization Plux inv-GFP</font><br><br>  
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   <td width="800px"><font size="5" face=verdana>Results</font><br>
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Revision as of 08:51, 27 October 2010




 

Lux promoter inverter

We've charaterized Plux inverter.
There is promoter (HSL-luxR repressor), biobrick number R0061.
We've made Plux inv-GFP and characterized about it.
Experiments

1.Preparing Plux inv-GFP

   cloning process is shown in Fig. 1

Fig. 1 cloning process

2.Characterization Plux inv-GFP

Results

Fig. 2 assay process


Reference


1) Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)

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