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Team:Chiba/lux promoter
From 2010.igem.org
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| - | <td width="800px"><font size="5" face=verdana>Lux promoter inverter</font | + | <td width="800px"><font size="5" face=verdana>Lux promoter inverter</font><br> |
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| - | <td width="800px"><font size="5" face=verdana>Experiments</font | + | <td width="800px"><font size="5" face=verdana>Experiments</font><br> |
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<font size="3">1,Preparing Plux inx-GFP</font> </font><br><br> | <font size="3">1,Preparing Plux inx-GFP</font> </font><br><br> | ||
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| - | <td width="800px"><font size="5" face=verdana>Results</font | + | <td width="800px"><font size="5" face=verdana>Results</font><br> |
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<font size="3">1,Preparing Plux inx-GFP</font> </font><br><br> | <font size="3">1,Preparing Plux inx-GFP</font> </font><br><br> | ||
Revision as of 08:14, 27 October 2010
| Lux promoter inverter We've charaterized Plux inverter. There is promoter (HSL-luxR repressor), biobrick number R0061. We've made Plux inv-GFP and characterized about it. |
| Experiments 1,Preparing Plux inx-GFP cloning process is shown in Fig. 1 |
Fig. 1 cloning process
| Results 1,Preparing Plux inx-GFP cloning process is shown in Fig. 1 |
Fig. 2 assay process
1) Egland.K.A, and Greenberg.E.P, Conversion of the Vibrio Fischeri Transcriptional Activator,LuxR, to a Repressor, J. Bacteriol., 182, P.805-811 (2000)
2) Cox.R.S.3rd, Surette.M.G, Elowitz.M.B, Programming gene expression with combinatorial promoters, Mol Syst Biol, 3 ,145 (2007)
