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Team:Chiba/Notebook 3
From 2010.igem.org
(Difference between revisions)
| Line 189: | Line 189: | ||
<pre>transformation | <pre>transformation | ||
| - | BBa_R0061 | + | strain:XL10-G |
| - | BBa_E0240 | + | Plasmid:BBa_R0061 |
| + | BBa_E0240 | ||
</pre> | </pre> | ||
<br> | <br> | ||
Revision as of 05:13, 27 October 2010
2010-10-8
transformation
strain:XL10-G
Plasmid:BBa_R0061
BBa_E0240
2010-10-9
Mini-prep
BBa_R0061
BBa_E0240
digestion
--------------------
BBa_R0061 20 µL
10×Buffer 3 µL
SpeI 1 µL
PstI 1 µL
NFW 5 µL
--------------------
30 µL
--------------------
BBa_E0240 20 µL
10×Buffer 3 µL
XbaI 1 µL
PstI 1 µL
NFW 5 µL
--------------------
30 µL
↓
gel extraction
2010-10-11
Ligation
-----------------------
BBa_R0061 (70 ng) 1 µL
BBa_E0240 (100 ng) 2 µL
ATP (10 mM) 1 µL
10×Buffer 1 µL
T4 DNA Ligase 1 µL
NFW 4 µL
-----------------------
10 µL
↓
transformation
2010-10-12
Ligation
-----------------------
BBa_R0061(100 ng) 1 µL
BBa_E0240 (100 ng) 3 µL
ATP (10 mM) 1 µL
T4 DNA Ligase 1 µL
dH<sub>2</sub>O 4 µL
-----------------------
10 µL
↓
transformation
strain:XL10-Gold
2010-10-21
Double transformation Strain:DH10B Plasmid:Plux inv-GFP & Plac-LuxR
2010-10-22
liquid culture LB (Carb-Kan) 1mL 37℃ 12 h 200 rpm
