Team:Chiba/Modeling

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{| style="color:#dd0000;background-color:#eeeeee;" cellpadding="3" cellspacing="1" border="1" bordercolor="#cc0303" width="62%" align="center"
 
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!align="center"|[[Team:Chiba|Home]]
 
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!align="center"|[[Team:Chiba/Team|Team]]
 
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!align="center"|[[Team:Chiba/Project|Project]]
 
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!align="center"|[[Team:Chiba/Parts|Parts]]
 
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!align="center"|[[Team:Chiba/Modeling|Modeling]]
 
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!align="center"|[[Team:Chiba/Safety|Safety]]
 
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[[Image:Chiba_model_1.jpg‎]]
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First, we use AHL for input and regard injection of AHL as clicking. <br>
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LuxR is generated constitutive.<br>
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Injecting AHL to Double Click Bacteria brings dimer of AHL and luxR.<br>
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We take lux promoter repressed by the dimer.<br>
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As the dimer represses Plux,<br>
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transcription of proteins under the Plux is stopped.<br>
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So in our project, <br>
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<b><font color="red">CLICK controls transcription of overall DNA sequence.</font></b>
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Second,there is a cI promoter above gfp DNA sequence.<br>
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cI works as repressor.<br>
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You can see cI DNA sequence is under lux promoter.<br>
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As AHL come into Double Click Bacteria, generation of cI is stopped.<br>
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Therefore, repressor of cI promoter disappears.<br>
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(Because the cause of repress(cI) which is kind of protein will be decomposed.)<br>
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If there isn't cI, bacteria shine with gfp.<br>
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We can say  <b><font color="red">cI recognizes the existence of input in this DNA sequence.</font></b>
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Third, there is AND Gate with T7ptag and supD.<br>
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As you know, T7ptag becomes T7 polymerase in case of existing supD.<br>
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T7 polymerase is translated to T7 protein and T7 works as activator of T7 promoter.<br>
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There is a T7 promoter above gfp DNA sequence.<br>
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If T7 have been generated once, <br>
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the T7 promoter starts translating and we can see bacteria shine with gfp.<br>
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On initial state(there isn't any click), bacteria don't have supD.<br>
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So there isn't any T7 polymerase and gfp cannot be genernated, in spite of being T7ptag in bacteria.<br>
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T7ptag which is a kind of mRNA is decomposed so fast.<br>
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However supD under lacI promoter is generated late.<br>
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<li><a href="https://2010.igem.org/Team:Chiba"><span>Home</span></a></li>
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Because, transcription of supD starts working after most of lacI are decomposed.<br>
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<li><a href="https://2010.igem.org/Team:Chiba/Team"><span>Team</span></a>
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<ul style="z-index:1"> <br>
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Team">Team</a></li> <li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Member">Member</a></li>
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After 1st click, supD exist.(supD is a kind of tRNA which is stable.)<br>
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/School">School</a></li>
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</li>
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There isn't input anymore, promoter above T7ptag DNA sequence starts activating.<br>
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a>
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<ul style="z-index:1"><br>
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                        <li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Project">Overall Project</a></li>
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As a result of reacting T7ptag and supD, the AND Gate is going to be available.<br>  
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_1">Circuit 1</a></li>
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Circuit_2">Circuit 2</a></li>
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In this case, the AND Gate remembers that  <b><font color="red">there was a click.</font></b><br>
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<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/Modeling"><span>Modeling</span></a></li>
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Moreover,
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<li><a href="https://2010.igem.org/Team:Chiba/Result"><span>Result</span></a>
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<br><br><br>
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<ul style="z-index:1"><br>
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                        <li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid1">Plasmid 1</a></li>
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[[Image:Chiba_model_4.jpg‎]]
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Plasmid2">Plasmid 2</a></li>
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/lux_promoter">lux Promoter</a></li><br>
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Actually, there is a hybrid promoter(cI/T7) above gfp DNA sequence.<br>
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<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a>
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                        <li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook">Notebook 1</a></li>
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T7 works as activator and cI works as repressor to the promoter.<br>
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook_2">Notebook 2</a></li>
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<li><a class="tablinks" href="https://2010.igem.org/Team:Chiba/Notebook_3">Notebook 3</a></li><br>
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</ul>
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<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li>
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<li><a href="https://2010.igem.org/Team:Chiba/Safety"><span>Safety</span></a></li>
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Latest revision as of 04:44, 25 October 2010




 




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