Team:Calgary/24 June 2010

From 2010.igem.org

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'''Thursday June 24, 2010'''
'''Thursday June 24, 2010'''
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[[Image:06.24.2010.E0040+B0015RestrictionDigest(2).jpg|thumb|400px|Dev's gel of restriction digest of E0040+B0015]]
<u>Emily</u>
<u>Emily</u>
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Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the  
Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the  
4000-3000 range which could be uncut plasmids
4000-3000 range which could be uncut plasmids
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[[Image:06.24.2010.E0040+B0015RestrictionDigest(2).jpg|thumb|400px|Dev's gel of restriction digest of E0040+B0015]]
 
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Revision as of 16:02, 12 July 2010

Thursday June 24, 2010

Dev's gel of restriction digest of E0040+B0015

Emily

Today primers came in for the maltose binding protein. After diluting them, we set up a PCR with a variety of different colonies in order to try to PCR this gene about of the genome of E Coli.


Patrick

The previous day's R0040-E0430 and R0040-E0420 constructs grew colonies, but there was no fluorescence visible. I will leave this for another day just to see. As well, I selected five colonies from each plate of cpxP in pSB1A3. They have been put into an overnight culture for miniprepping tomorrow.


Dev

Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the 4000-3000 range which could be uncut plasmids

No notebook page exists for this date. Sorry!