Team:Calgary/22 August 2010

From 2010.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 3: Line 3:
Weekends Rule!!
Weekends Rule!!
 +
 +
[[Image:08.21.2010 ColonyPCRCE..jpg|thumb|400px|Colony PCR agarose gel electrophoresis run on 1.0% at 90V for 45 minutes. Lanes 1 and 2 are K239000-I13507, Lanes 3-6 are K239000-I13504, Lanes 7-16 are I0500-I13507, and Lanes 17 and 18 are Controls with no DNA]]
 +
<u>Chris</u>
 +
 +
Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow.
 +
 +
<u>Emily</u>
 +
 +
Today I ran gels of the two remaining colony PCR's from yesterday: one of the putitive I0500-B0034-malE31-cpxR-I13507 circuit as well as the IO500-B0034-malE31 circuit in cpxR competant cells.
}}
}}

Latest revision as of 05:02, 24 August 2010

Sunday August 22, 2010

Weekends Rule!!

Colony PCR agarose gel electrophoresis run on 1.0% at 90V for 45 minutes. Lanes 1 and 2 are K239000-I13507, Lanes 3-6 are K239000-I13504, Lanes 7-16 are I0500-I13507, and Lanes 17 and 18 are Controls with no DNA

Chris

Today, I put digests of CpxP to insert into the AK plasmid. Emily and I also spent time defrosting the freezer. I did plasmid preparations of NLPE, Controls and MalE with Alex. Finally, I did ligations of CpxP into pSB1AK3, MalE31 into pSB2K3, and ibpAB into pSB1AK3. The plates will be seen tomorrow.

Emily

Today I ran gels of the two remaining colony PCR's from yesterday: one of the putitive I0500-B0034-malE31-cpxR-I13507 circuit as well as the IO500-B0034-malE31 circuit in cpxR competant cells.