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From 2010.igem.org

The CBNU 2009 iGEM team, our team's goal was to manage and retrieve required data and explore informations through mapping essential genes into orthologs of interesting genomes and through statistical analyses of gene order and direction, so on.

Previous iGEM teams have planned about making synthetic gemone for synthetic cell and programe to design the minimal genome.

We managed and retrieved required data and explored informations through mapping essential gens into orthologs of interesting genomes and through statistical analyses of gene order and direction, so on.

 

Now we are trying putting the plan in practice. Our final main purpose is synthesis a synthetic cell. If you want synthesis a synthetic cell, you need a minimal genome.

The minimal genome is the smallest possible group of genes that would be sufficient to sustain a cellular life form under the most favorable conditions imaginable. There are two ways to synthesize a minimal genome, which are top-down and bottom-up approaches. We are interested in the bottom-up approach, in which the major hurdle is how to degine the minimal genome. We don't know the principle of the organization of the genome. Moreover to verify the design experimentally, we should to deal with some 200 or more essential genes. So, we use the divide-and-conquer strategy to solve the problems in designing the minimal genome.

We will make E.coli having two chromosomes. One is a normal chromosome and the other is a normal chromosome and the other is a model minimal chromosomes, in which the replication and segregation processes and their regulation are occurred independently. Studies on the minimal chromosome will provide us useful information about designing the minimal genome.

This project's purpose is design and making of Minimal Synthetic Chromosome(MSC). Then we need this step for successful process.

(1) Methodical classification of bacterial chromosome copy and division

(2) Development of oriC-finder program for finding Replication Origin of Chromosome(oriC)

(3) Making Bacterial chromosome copy and Division database

(4) Stepwise production and investigation of Minimal Synthetic Chromosome(MSC)