Team:CBNU-Korea

From 2010.igem.org

(Difference between revisions)
 
(125 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Template:CBNU-Korea}}
 +
<html>
<html>
-
<head>
 
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8"/>
 
-
<title>Transport template</title>
 
-
<link rel="stylesheet" href="style.css" type="text/css" charset="utf-8" />
 
-
 
-
</head>
 
-
 
-
<style>
 
-
 
-
html, body, h1, h2, h3, h4 {
 
-
margin: 0;
 
-
padding: 0;
 
-
}
 
-
h1 img {
 
-
display: block;
 
-
}
 
-
img {
 
-
border: 0;
 
-
}
 
-
a {
 
-
color: #464544;
 
-
}
 
-
a:hover {
 
-
color: #666666;
 
-
}
 
-
.left {
 
-
float: left;
 
-
}
 
-
.right {
 
-
float: right;
 
-
}
 
-
.more {
 
-
text-align: right;
 
-
}
 
-
.clear {
 
-
clear: both;
 
-
}
 
-
 
-
body {
 
-
background: url(https://static.igem.org/mediawiki/2010/f/ff/Page_bg.gif);
 
-
text-align: center;
 
-
font: 12px arial, sans-serif;
 
-
color: #464544;
 
-
padding-bottom: 10px;
 
-
}
 
-
 
-
#outer {
 
-
  background: top left url( /////////////////////////////////////////            ) repeat-x;
 
-
  padding-top: 20px;
 
-
}
 
-
 
-
/** layout **/
 
-
#wrapper {
 
-
text-align: left;
 
-
margin: auto;
 
-
width: 875px;
 
-
  position: relative;
 
-
  background: url(///////////////////////////////////////////////);
 
-
}
 
-
#body-bot {
 
-
  background: bottom left url(https://static.igem.org/mediawiki/2010/7/7a/Bg_down_with_mark.jpg) no-repeat;
 
-
}
 
-
#body-top {
 
-
  background: url(https://static.igem.org/mediawiki/2010/0/01/Sa.jpg) no-repeat;
 
-
  min-height: 873px;
 
-
  _height: 481px;
 
-
  padding-bottom: 14px;
 
-
}
 
-
 
-
/** logo **/
 
-
 
-
#logo {
 
-
  width: 25em;
 
-
  text-align: center;
 
-
}
 
-
#logo h1 {
 
-
  color: #6F7033;
 
-
  font: 22px "Arial narrow", arial, sans-serif;
 
-
  line-height: 1;
 
-
  padding-top: 0.8em;
 
-
}
 
-
#logo p {
 
-
  color: #464544;
 
-
  font: 16px "Arial narrow", arial, sans-serif;
 
-
  margin: 0;
 
-
}
 
-
 
-
/** nav **/
 
-
 
-
#nav {
 
-
  margin-top: 50px;
 
-
}
 
-
#nav li {
 
-
  list-style: none;
 
-
  float: left;
 
-
  background: left center url(https://static.igem.org/mediawiki/2010/5/54/Arr.gif) no-repeat;
 
-
  padding-left: 15px;
 
-
  padding-right: 20px;
 
-
}
 
-
#nav a {
 
-
  color: #000;
 
-
  text-decoration: none;
 
-
  font-weight: bold;
 
-
}
 
-
#nav a:hover {
 
-
  color: #FFA405;
 
-
}
 
-
 
-
/** green box **/
 
-
#gbox {
 
-
  width: 431px;
 
-
  margin-top: 47px;
 
-
  margin-left: 22px;
 
-
}
 
-
 
-
#gbox-bg  { background: url(https://static.igem.org/mediawiki/2010/0/01/Green_bg.gif) repeat-y; }
 
-
#gbox-grd { background: bottom url(https://static.igem.org/mediawiki/2010/5/51/Green_grad_bot.jpg) repeat-x; }
 
-
#gbox-top { background: url(https://static.igem.org/mediawiki/2010/5/50/Green_top.gif) no-repeat; height: 24px; }
 
-
#gbox-bot { background: bottom left url(https://static.igem.org/mediawiki/2010/6/61/Green_bot.gif) no-repeat; height: 23px; }
 
-
 
-
#gbox-grd {
 
-
  min-height: 300px;
 
-
  padding: 2px 24px;
 
-
}
 
-
 
-
#gbox h2 {
 
-
  font: bold 14px arial, sans-serif;
 
-
  color: #000;
 
-
  margin: 0;
 
-
}
 
-
 
-
#gbox p {
 
-
  margin: 1em 0;
 
-
}
 
-
 
-
#features {
 
-
  background: #EDF0C4;
 
-
  padding: 14px;
 
-
  margin: 0 -14px;
 
-
}
 
-
#features h2 {
 
-
  position: relative;
 
-
}
 
-
#features ul {
 
-
  float: left;
 
-
  width: 49%;
 
-
  margin: 1em 0;
 
-
  padding: 0;
 
-
}
 
-
#features li {
 
-
  list-style: square;
 
-
  margin-left: 12px;
 
-
}
 
-
 
-
/** footer **/
 
-
 
-
#newsletter, #events {
 
-
  float: left;
 
-
  width: 49%;
 
-
  margin-top: 1em;
 
-
}
 
-
#newsletter h2, #events h2 {
 
-
  color: #fff;
 
-
}
 
-
#newsletter .text {
 
-
  width: 125px;
 
-
}
 
-
#newsletter form {
 
-
  margin: 0.3em 0 0.6em 0;
 
-
}
 
-
#newsletter p {
 
-
  margin: 0.3em 0;
 
-
  font-size: 10px;
 
-
}
 
-
#newsletter a {
 
-
  color: #000;
 
-
}
 
-
 
-
#events {
 
-
  margin-left: 6%;
 
-
  width: 38%;
 
-
}
 
-
#events ul {
 
-
  margin: 0.3em 0;
 
-
  padding: 0;
 
-
}
 
-
#events li {
 
-
  list-style: none;
 
-
  margin: 0;
 
-
  padding: 0;
 
-
}
 
-
 
-
/** grey box **/
 
-
 
-
#greybox {
 
-
  background: url(https://static.igem.org/mediawiki/2010/c/ce/Grey_bg.gif) repeat-y;
 
-
  width: 326px;
 
-
  position: absolute;
 
-
  top: 292px;
 
-
  left: 457px;
 
-
}
 
-
#greybox-top {
 
-
  background: url(////////////////////////////////////////////////////////////////////) no-repeat;
 
-
  padding-left: 165px;
 
-
  padding-top: 18px;
 
-
}
 
-
#greybox-bot {
 
-
  background: bottom left url(https://static.igem.org/mediawiki/2010/a/af/Grey_bot.gif) no-repeat;
 
-
  padding-bottom: 4px;
 
-
}
 
-
#greybox h2 {
 
-
  font: bold 12px arial, sans-serif;
 
-
  color: #E4EE6A;
 
-
  line-height: 1.5;
 
-
}
 
-
#greybox a {
 
-
  color: #fff;
 
-
}
 
-
 
-
#copyright {
 
-
  font-size: 9px;
 
-
  color: #fff;
 
-
  margin-top: 60px;
 
-
}
 
-
 
-
</style>
 
<body>
<body>
-
 
+
<p align="center">&nbsp;</p>
-
  <div id="outer">
+
<p align="center"><img src="https://static.igem.org/mediawiki/2010/0/02/Usynb_logo.png" /></p>
-
    <div id="wrapper">
+
<p align="center">2010 CBNU-KOREA iGEM team’s final goal is  minimal genome synthesis and synthetic cell.</p>
-
      <div id="body-bot">
+
<p align="center"> But synthesizing all these is very  complicate and too many things we have to know. </p>
-
        <div id="body-top">
+
<p align="center">For example, thing like how to  decide genes to organize and arrange in minimal genome or select what kind of  genes we use.</p>
-
          <div id="logo">
+
<p align="center">So, as the first step of our ultimate goal, we synthesized  minimal chromosome. </p>
-
            <h1>Korea Chungbuk National University</h1>
+
<p align="center"><br />
-
            <p>SYNB Microbial Genetics Lab</p>
+
  In  order to synthesize the minimal chromosome, we used some essential genes,  particular sites</p>
-
          </div>
+
<p align="center"> and origin of <em>V.cholerae</em> O1 biovar eltor str. N16961 chromosome II, <em>parA,  parB, rctA, rctB</em> protein coding genes,<em>parS,  dif</em> sites </p>
-
          <div id="nav">
+
<p align="center">and origin. For confirming it’s function is working well, we  employ GFP protein and fusion to ParB protein and then observe fluorescence  microscope. </p>
-
            <ul>
+
<p align="center"><br />
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea">HOME</a></li>
+
  Also,  we reconstruct essential gene database from DEG and NCBI.</p>
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea/Team">TEAM</a></li>
+
<p align="center"> And we make Genome  Designer using this reconstructed database and add it to Artemis’s DNADraw.</p>
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea/Project">PROJECT</a></li>
+
<div align="center">
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea/Parts">PARTS</a></li>
+
  <p><img src="https://static.igem.org/mediawiki/2010/4/4e/01.jpg" alt="" width="371" height="106" /></p>
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea/Modeling">MODELLING</a></li>
+
  <p>   <img src="https://static.igem.org/mediawiki/2010/9/97/Boineer_Logo.jpg" width="330" height="60"></p>
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea/Notebook">NOTEBOOK</a></li>
+
</div>
-
              <li><a href="https://2010.igem.org/Team:CBNU-Korea/Safety">SAFETY</a></li>
+
-
            </ul>
+
-
            <div class="clear"> </div>
+
-
          </div>
+
-
          <div id="gbox">
+
-
            <div id="gbox-top"> </div>
+
-
            <div id="gbox-bg">
+
-
              <div id="gbox-grd">
+
-
                <h2>WELCOME TO OUR TRUCK TRACKING COMPANY</h2>
+
-
+
-
                <p>Don't forget to check <a href="http://www.freewebsitetemplates.com">free website templates</a> every day, because we add a new free website template almost daily.</p>
+
-
          <p>You can remove any link to our websites from this template you're free to use the template without linking back to us.</p>
+
-
                <p>This is just a place holder so you can see how the site would look like.</p>
+
-
 
+
-
<p>This is a template designed by free website templates for you for free you can replace all the text by your own text.</p>
+
-
                <div id="features">
+
-
                  <h2>협찬사</h2>
+
-
                  <ul>
+
-
                    <li><a href="http://www.freewebsitetemplates.com">Free Website Templates</a></li>
+
-
<li><a href="http://www.freewebsitetemplates.com/forum/">The forum</a></li>
+
-
                    <li><a href="http://www.justwebtemplates.com">Just Web Templates</a></li>
+
-
                    <li><a href="http://www.templatebeauty.com">Template Beauty</a></li>
+
-
                   
+
-
                  </ul>
+
-
                  <ul>
+
-
  <li><a href="http://www.templatebeauty.com">Template Beauty</a></li>
+
-
                    <li><a href="http://www.freewebsitetemplates.com">Free Website Templates</a></li>
+
-
<li><a href="http://www.freewebsitetemplates.com/forum/">The forum</a></li>
+
-
                    <li><a href="http://www.justwebtemplates.com">Just Web Templates</a></li>
+
-
                  </ul>
+
-
                  <div class="clear"> </div>
+
-
                </div>
+
-
               
+
-
                                <div class="clear"> </div>
+
-
               
+
-
              </div>
+
-
            </div>
+
-
            <div id="gbox-bot"> </div>
+
-
          </div>
+
-
                  </div>
+
-
      </div>
+
-
    </div>
+
-
  </div>
+
-
 
+
-
  <div id="copyright">
+
-
    &copy; Copyright information goes here. All rights reserved.
+
-
  </div>
+
-
 
+
</body>
</body>
</html>
</html>

Latest revision as of 03:58, 28 October 2010

 

2010 CBNU-KOREA iGEM team’s final goal is minimal genome synthesis and synthetic cell.

But synthesizing all these is very complicate and too many things we have to know.

For example, thing like how to decide genes to organize and arrange in minimal genome or select what kind of genes we use.

So, as the first step of our ultimate goal, we synthesized minimal chromosome.


In order to synthesize the minimal chromosome, we used some essential genes, particular sites

and origin of V.cholerae O1 biovar eltor str. N16961 chromosome II, parA, parB, rctA, rctB protein coding genes,parS, dif sites

and origin. For confirming it’s function is working well, we employ GFP protein and fusion to ParB protein and then observe fluorescence microscope.


Also, we reconstruct essential gene database from DEG and NCBI.

And we make Genome Designer using this reconstructed database and add it to Artemis’s DNADraw.