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From 2010.igem.org

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  • Light-Pattern Control
  • E. Cargo (Tat-PTD)
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Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.

PCR

• PCR

PCR Cleanup/Purification

• PCR Cleanup/Purification PDF (page 19)

Gel Electrophoresis

• Agarose gels

Gel Extraction

• Gel Extraction PDF (page 25)

Ligations

• Ligation

Restriction digests

• Restriction [Double] Digest

Overnights

• Overnight Culture

Transformations

• Bacterial Cell Transformation

Cell competence protocols

• Quick CaCL2 Competence PDF
• CaCl2 competence (bulk) PDF

Minipreps

• Miniprep handbook PDF (pages 22-23)