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Team:Brown/Parts
From 2010.igem.org
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<groupparts>iGEM010 Brown</groupparts> | <groupparts>iGEM010 Brown</groupparts> | ||
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| + | ===Part:BBa_K324000 - LacI repressor protein in RFC25 format=== | ||
| + | This part consists of the lacI repressor protein (BBa_I732100) optimized with endings for protein fusion in the Freiburg assembly format (RFC25). There is also a 6-his tag appended to the C-terminus for easy purification using Ni-affinity column. RFC 25 prefix/suffix and 6-his sequences were attached via PCR. | ||
| + | ====Design Notes==== | ||
| + | 6-his tag was appended to the 3' end of this protein to allow for purification upon expression in E. coli. We chose a C-terminal tag to avoid isolation of incompletely translated peptides. | ||
| + | ====Source==== | ||
| + | [http://partsregistry.org/Part:BBa_I732100] Submitted by the USTC '07 iGEM team, taken from the 2010 Spring Distribution | ||
Revision as of 02:02, 27 October 2010
BioBricks
<groupparts>iGEM010 Brown</groupparts>
Part:BBa_K324000 - LacI repressor protein in RFC25 format
This part consists of the lacI repressor protein (BBa_I732100) optimized with endings for protein fusion in the Freiburg assembly format (RFC25). There is also a 6-his tag appended to the C-terminus for easy purification using Ni-affinity column. RFC 25 prefix/suffix and 6-his sequences were attached via PCR.
Design Notes
6-his tag was appended to the 3' end of this protein to allow for purification upon expression in E. coli. We chose a C-terminal tag to avoid isolation of incompletely translated peptides.
Source
[http://partsregistry.org/Part:BBa_I732100] Submitted by the USTC '07 iGEM team, taken from the 2010 Spring Distribution
